Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets

Transmissible gastroenteritis coronavirus (TGEV) is one of the most severe threats to the swine industry. In this study, we constructed a suite of recombinant Lactobacillus plantarum with surface displaying the spike (S) protein coming from TGEV and fused with DC cells targeting peptides (DCpep) to...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2018-10, Vol.102 (19), p.8403-8417
Main Authors: Jin, Yu-Bei, Yang, Wen-Tao, Shi, Chun-Wei, Feng, Bo, Huang, Ke-Yan, Zhao, Guang-Xun, Li, Qiong-Yan, Xie, Jing, Huang, Hai-Bin, Jiang, Yan-Long, Wang, Jian-Zhong, Wang, Guan, Kang, Yuan-Huan, Yang, Gui-Lian, Wang, Chun-Feng
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - immunology
Applied Genetics and Molecular Biotechnology
B cells
B-Lymphocytes - immunology
Bacteria
Bacterial Proteins - immunology
Biomedical and Life Sciences
Biotechnology
CD3 antigen
CD4 antigen
CD80 antigen
Coronaviridae
Coronaviruses
Gastroenteritis
Gastroenteritis, Transmissible, of Swine - immunology
Ileum
Immune response
Immunoglobulin A
Immunoglobulin A, Secretory - immunology
Immunoglobulin G
Immunoglobulin G - immunology
Intracellular Signaling Peptides and Proteins - immunology
Lactobacillus plantarum
Lactobacillus plantarum - immunology
Lamina propria
Life Sciences
Livestock
Lymphocytes
Lymphocytes B
Lymphocytes T
Major histocompatibility complex
Microbial Genetics and Genomics
Microbiology
Peptides
Pork industry
Proteins
Spike protein
Swine
T cells
T-Lymphocytes - immunology
Transmissible gastroenteritis
Transmissible gastroenteritis virus - immunology
Vaccines
Viral Vaccines - immunology
Viruses
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Summary:Transmissible gastroenteritis coronavirus (TGEV) is one of the most severe threats to the swine industry. In this study, we constructed a suite of recombinant Lactobacillus plantarum with surface displaying the spike (S) protein coming from TGEV and fused with DC cells targeting peptides (DCpep) to develop an effective, safe, and convenient vaccine against transmissible gastroenteritis. Our research results found that the recombinant Lactobacillus plantarum (NC8-pSIP409-pgsA-S-DCpep) group expressing S fused with DCpep could not only significantly increase the percentages of MHC-II + CD80 + B cells and CD3 + CD4 + T cells but also the number of IgA + B cells and CD3 + CD4 + T cells of ileum lamina propria, which elevated the specific secretory immunoglobulin A (SIgA) titers in feces and IgG titers in serum. Taken together, these results suggest that NC8-pSIP409-pgsA-S-DCpep expressing the S of TGEV fused with DCpep could effectively induce immune responses and provide a feasible original strategy and approach for the design of TGEV vaccines.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-018-9205-0