Plasma neutrophil extracellular trap level is modified by disease severity and inhaled corticosteroids in chronic inflammatory lung diseases

A flow cytometry-based method was developed to quantify in vivo circulating neutrophil extracellular trap (NET) levels in plasma and compare them in patients with different chronic inflammatory lung diseases. Seventeen asthmatic and 11 control children, 12 adult controls, 46 asthmatic, 6 COPD and 6...

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Bibliographic Details
Published in:Scientific reports 2020-03, Vol.10 (1), p.4320-4320, Article 4320
Main Authors: Gál, Zsófia, Gézsi, András, Pállinger, Éva, Visnovitz, Tamás, Nagy, Adrienne, Kiss, András, Sultész, Monika, Csoma, Zsuzsanna, Tamási, Lilla, Gálffy, Gabriella, Szalai, Csaba
Format: Article
Language:English
Subjects:
Administration, Inhalation
Adrenal Cortex Hormones - administration & dosage
Adult
Adults
Aged
Anti-Asthmatic Agents - administration & dosage
Asthma
Asthma - blood
Asthma - drug therapy
Asthma - pathology
Case-Control Studies
Child
Children
Chronic obstructive pulmonary disease
Corticosteroids
Extracellular Traps - drug effects
Extracellular Traps - metabolism
Female
Flow cytometry
Forced Expiratory Volume
Humans
Lung diseases
Male
Middle Aged
Neutrophils
Pulmonary Disease, Chronic Obstructive - blood
Pulmonary Disease, Chronic Obstructive - drug therapy
Pulmonary Disease, Chronic Obstructive - pathology
Respiratory therapy
Severity of Illness Index
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Summary:A flow cytometry-based method was developed to quantify in vivo circulating neutrophil extracellular trap (NET) levels in plasma and compare them in patients with different chronic inflammatory lung diseases. Seventeen asthmatic and 11 control children, 12 adult controls, 46 asthmatic, 6 COPD and 6 adult patients with asthma-COPD overlap syndrome (ACOS) were recruited in the study. The presence of NETs in unstimulated cell-free plasma was confirmed and visualized by confocal laser-scanning microscopy. No significant differences were found in plasma NET levels between children and adults, children with or without asthma and adults with or without asthma, COPD or ACOS. When asthmatic patients were stratified according to their disease severity the average plasma NET level was significantly higher in asthmatic patients with more serious symptoms (adjusted p = 0.027). Patients with poorer pulmonary functions had higher plasma NET levels which negatively correlated with the FEV1 values (r = -0.39, p = 0.002). Patients who were medicated daily with inhaled corticosteroids (ICS) had significantly lower average plasma NET level than patients who did not or just occasionally used ICS (p = 0.027). If further studies confirm the NET-lowering effect of ICS in the circulation, it can be utilized in diseases where NETosis contributes to the pathogenesis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-61253-2