Long non-coding RNA PSMA3-AS1 promotes malignant phenotypes of esophageal cancer by modulating the miR-101/EZH2 axis as a ceRNA

Emerging evidences has demonstrated that dysregulation of long non-coding RNAs (lncRNAs) is critically involved in esophageal squamous cell carcinoma (ESCC) progression. However, the function of lncRNA PSMA3-AS1 in ESCC is unclear. Therefore, we aimed to explore the functions and potential mechanism...

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Published in:Aging (Albany, NY.) NY.), 2020-01, Vol.12 (2), p.1843-1856
Main Authors: Qiu, Bai-Quan, Lin, Xia-Hui, Ye, Xu-Dong, Huang, Wei, Pei, Xu, Xiong, Dian, Long, Xiang, Zhu, Shu-Qiang, Lu, Feng, Lin, Kun, Zhang, Xiao-Qiang, Xu, Jian-Jun, Sheng, Lu-Lu, Zhang, Xue-Mei, Zhang, Peng-Fei, Wu, Yong-Bing
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Adult
Aged
Cell Line, Tumor
Cell Movement
Cell Proliferation
Enhancer of Zeste Homolog 2 Protein - genetics
Esophageal Neoplasms - genetics
Esophageal Neoplasms - pathology
Female
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs - genetics
Middle Aged
Neoplasm Metastasis
Neoplasm Staging
Proteasome Endopeptidase Complex - genetics
Research Paper
RNA Interference
RNA, Long Noncoding - genetics
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Summary:Emerging evidences has demonstrated that dysregulation of long non-coding RNAs (lncRNAs) is critically involved in esophageal squamous cell carcinoma (ESCC) progression. However, the function of lncRNA PSMA3-AS1 in ESCC is unclear. Therefore, we aimed to explore the functions and potential mechanisms of PSMA3-AS1 in ESCC cells progression. Here, we found that PSMA3-AS1 expression was significantly up-regulated in ESCC tissues. Forced PSMA3-AS1 expression was correlated with tumor size, distant metastasis, and poor prognosis in ESCC patients. Functionally, PSMA3-AS1-overexpression promoted ESCC cells proliferation, invasion, and migration in vitro. Mechanistically, PSMA3-AS1 up-regulated EZH2 expression by competitively binding to miR-101. PSMA3-AS1 is significantly up-regulated in ESCC tissues, and the PSMA3-AS1/miR-101/EZH2 axis plays a critical role in ESCC progression. Taken together, our results may provide promising targets for ESCC therapy. PSMA3-AS1 and miR-101 expression were explored using qRT-PCR in ESCC tissues and cell lines. Immunohistochemistry assays were carried out to analyze EZH2 (enhancer of zeste homolog) protein expression. RIP, dual-luciferase reporter, fluorescence in situ hybridization, and biotin pull-down assays were used to detect the interactions of PSMA3-AS1, miR-101 and EZH2. The biological functions of PSMA3-AS1 in PSMA3-AS1-altered cells were explored using CCK-8, colony formation, wound healing, and transwell assays in vitro.
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.102716