Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone

Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel strategy for enhancing the intrinsic potential of the brain to regenerate afte...

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Bibliographic Details
Published in:Molecular neurobiology 2020-02, Vol.57 (2), p.997-1008
Main Authors: Zhang, Zhichao, Manaf, Adeel, Li, Yanjiao, Perez, Sonia Peña, Suganthan, Rajikala, Dahl, John Arne, Bjørås, Magnar, Klungland, Arne
Format: Article
Language:eng ; nor
Subjects:
ASCL1 protein
Biomedical and Life Sciences
Biomedicine
Brain injury
Cell Biology
Doublecortin protein
Epigenetics
Histone H3
Lysine
Methylation
Neural stem cells
Neurobiology
Neurogenesis
Neurology
Neurosciences
Progenitor cells
Subventricular zone
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Summary:Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel strategy for enhancing the intrinsic potential of the brain to regenerate after brain injury. Therefore, defining the epigenetic features of NSPCs is important for developing epigenetic therapies for targeted reprogramming of NSPCs to rescue neurologic function after injury. In this study, we aimed at defining different subtypes of NSPCs by individual histone methylations. We found the three histone marks, histone H3 lysine 4 trimethylation (H3K4me3), histone H3 lysine 27 trimethylation (H3K27me3), and histone H3 lysine 36 trimethylation (H3K36me3), to nicely and dynamically portray individual cell types during neurodevelopment. First, we found all three marks co-stained with NSPC marker SOX2 in mouse subventricular zone. Then, CD133, Id1, Mash1, and DCX immunostaining were used to define NSPC subtypes. Type E/B, B/C, and C/A cells showed high levels of H3K27me3, H3K36me3, and H3K4me3, respectively. Our results reveal defined histone methylations of NSPC subtypes supporting that epigenetic regulation is critical for neurogenesis and for maintaining NSPCs.
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-019-01777-5