3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnet...

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Bibliographic Details
Published in:Scientific reports 2020-02, Vol.10 (1), p.2289, Article 2289
Main Authors: Goranov, V, Shelyakova, T, De Santis, R, Haranava, Y, Makhaniok, A, Gloria, A, Tampieri, A, Russo, A, Kon, E, Marcacci, M, Ambrosio, L, Dediu, V A
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Bone Regeneration
Cell growth
Clinical medicine
Computer Simulation
Fibers
Flow cytometry
Human Umbilical Vein Endothelial Cells - physiology
Humans
Magnetic Fields
Magnetite Nanoparticles - chemistry
Materials Testing
Mathematical models
Mesenchymal Stem Cells - physiology
Microscopy
Models, Biological
Models, Chemical
Nanomedicine - methods
Nanoparticles
Nanostructured materials
Neovascularization, Physiologic - physiology
Osteogenesis - physiology
Osteoprogenitor cells
Pattern formation
Proof of Concept Study
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-58738-5