Induction of Osmoadaptive Mechanisms and Modulation of Cellular Physiology Help Bacillus licheniformis Strain SSA 61 Adapt to Salt Stress

Bacillus licheniformis strain SSA 61, originally isolated from Sambhar salt lake, was observed to grow even in the presence of 25 % salt stress. Osmoadaptive mechanisms of this halotolerant B. licheniformis strain SSA 61, for long-term survival and growth under salt stress, were determined. Proline...

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Bibliographic Details
Published in:Current microbiology 2015-04, Vol.70 (4), p.610-617
Main Authors: Paul, Sangeeta, Aggarwal, Chetana, Thakur, Jyoti Kumar, Bandeppa, G. S., Khan, Md. Aslam, Pearson, Lauren M., Babnigg, Gyorgy, Giometti, Carol S., Joachimiak, Andrzej
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Anatomy & physiology
Ascorbic Acid - analysis
Bacillus - drug effects
Bacillus - physiology
Bacillus licheniformis
Bacteria
Biomedical and Life Sciences
Biotechnology
Cellular biology
Freshwater
Gene Expression Profiling
Glutathione - analysis
Lakes
Life Sciences
Microbial Viability - drug effects
Microbiology
Osmotic Pressure
Proline - analysis
Salt
Salts - metabolism
Stress analysis
Stress, Physiological
Superoxide Dismutase - metabolism
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Summary:Bacillus licheniformis strain SSA 61, originally isolated from Sambhar salt lake, was observed to grow even in the presence of 25 % salt stress. Osmoadaptive mechanisms of this halotolerant B. licheniformis strain SSA 61, for long-term survival and growth under salt stress, were determined. Proline was the preferentially accumulated compatible osmolyte. There was also increased accumulation of antioxidants ascorbic acid and glutathione. Among the different antioxidative enzymes assayed, superoxide dismutase played the most crucial role in defense against salt-induced stress in the organism. Adaptation to stress by the organism involved modulation of cellular physiology at various levels. There was enhanced expression of known proteins playing essential roles in stress adaptation, such as chaperones DnaK and GroEL, and general stress protein YfkM and polynucleotide phosphorylase/polyadenylase. Proteins involved in amino acid biosynthetic pathway, ribosome structure, and peptide elongation were also overexpressed. Salt stress-induced modulation of expression of enzymes involved in carbon metabolism was observed. There was up-regulation of a number of enzymes involved in generation of NADH and NADPH, indicating increased cellular demand for both energy and reducing power.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-014-0761-y