Deletion of Ck2β gene causes germ cell development arrest and azoospermia in male mice

Objectives In humans, non‐obstructive azoospermia (NOA) is a major cause of male infertility. However, the aetiology of NOA is largely unknown. Previous studies reported that protein CK2β was abundantly and broadly expressed in spermatogenic cells. Here, we investigate whether protein CK2β participa...

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Bibliographic Details
Published in:Cell proliferation 2020-01, Vol.53 (1), p.e12726-n/a
Main Authors: Liang, Qiu‐Xia, Wang, Zhen‐Bo, Lei, Wen‐Long, Lin, Fei, Qiao, Jing‐Yi, Filhol‐Cochet, Odile, Boldyreff, Brigitte, Schatten, Heide, Sun, Qing‐Yuan, Qian, Wei‐Ping
Format: Article
Language:English
Subjects:
Ablation
Animal biology
Animals
Antibodies
Apoptosis
Apoptosis - genetics
azoospermia
Azoospermia - enzymology
Azoospermia - genetics
Azoospermia - pathology
Casein Kinase II - deficiency
Casein Kinase II - metabolism
cell apoptosis
Cell survival
CK2β
Deactivation
Deoxyribonucleic acid
Development Biology
DNA
DNA damage
Ethanol
Fertility
Gene Deletion
Gene expression
Germ Cells - enzymology
Germ Cells - pathology
Immunoblotting
Immunofluorescence
Immunohistochemistry
Inactivation
Infertility
Kinases
Life Sciences
Male
Mice
Mice, Knockout
Molecular modelling
Mutation
Original
Pattern analysis
Phenotypes
Proteins
Rodents
Sedimentation
Spermatogenesis
Statistical analysis
Testes
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Summary:Objectives In humans, non‐obstructive azoospermia (NOA) is a major cause of male infertility. However, the aetiology of NOA is largely unknown. Previous studies reported that protein CK2β was abundantly and broadly expressed in spermatogenic cells. Here, we investigate whether protein CK2β participates in spermatogenesis. Materials and Methods In this study, we separated spermatogenic cells using STA‐PUT velocity sedimentation, analysed the expression pattern of protein CK2β by immunoblotting, specifically deleted Ck2β gene in early‐stage spermatogenic cells by crossing Ck2βfl mice with Stra8‐Cre+ mice and validated the knockout efficiency by quantitative RT‐PCR and immunoblotting. The phenotypes of Ck2βfl/Δ;SCre+ mice were studied by immunohistochemistry and immunofluorescence. The molecular mechanisms of male germ cell development arrest were elucidated by immunoblotting and TUNEL assay. Results Ablation of Ck2β gene triggered excessive germ cell apoptosis, germ cell development arrest, azoospermia and male infertility. Inactivation of Ck2β gene caused distinctly reduced expression of Ck2α′ gene and CK2α′ protein. Conclusions Ck2β is a vital gene for germ cell survival and male fertility in mice.
ISSN:0960-7722
1365-2184
DOI:10.1111/cpr.12726