A simple and accurate PCR method for detection of genetically modified rice

Background Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people’s food...

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Bibliographic Details
Published in:Journal of environmental health science and engineering 2019-12, Vol.17 (2), p.847-851
Main Authors: Safaei, Payam, Aghaee, Ebrahim Molaee, Khaniki, Gholamreza Jahed, Afshari, Setareh Agha Kuchak, Rezaie, Sassan
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
Earth and Environmental Science
Environment
Environmental Economics
Environmental Engineering/Biotechnology
Environmental Health
Environmental Law/Policy/Ecojustice
Genes
Genetic aspects
Genetic modification
Genetic research
Genetically engineered foods
Genetically modified organisms
Health
Legislation
Methods
Nitric-oxide synthase
Phosphates
Polymerase chain reaction
Quality of Life Research
Research Article
Rice
Sucrose
Sucrose phosphate synthase
Sugar
Target detection
Testing
Viruses
Waste Management/Waste Technology
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Summary:Background Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people’s food diet. Methods In this study, eighty-one non-labeled rice samples were collected randomly from different market sites of Tehran, Iran. In order to analysis, rice genomic DNA was extracted using MBST DNA extraction kit and subsequently, sucrose phosphate synthase (SPS) gene was used to confirm the quality of extracted DNA. Then, cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium nopaline synthase (NOS) terminator were selected as screening targets for detection of GM rice sequences by PCR. Results According to our results, 2 out of 81 (2.4%) samples tested were positive for CaMV 35S promoter while no positive result was detected for NOS terminator. Conclusion The obtained data indicated that this method is capable to identify the GM rice varieties. Furthermore, it can demonstrate the possibility of the presence of GM rice in Tehran’s market, thus putting emphasis on the requirement for developing a precise approach to evaluate this product.
ISSN:2052-336X
2052-336X
DOI:10.1007/s40201-019-00401-x