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Virulence of Pseudomonas aeruginosa exposed to carvacrol: alterations of the Quorum sensing at enzymatic and gene levels
The main goal of this study was to evaluate the inhibition of Pseudomonas aeruginosa virulence factors and Quorum Sensing during exposure to carvacrol. P. aeruginosa (ATCC 10154) was exposed to carvacrol determining changes in biofilm development, motility, acyl-homoserine lactones (AHL) synthesis a...
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Published in: | Journal of cell communication and signaling 2019-12, Vol.13 (4), p.531-537 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The main goal of this study was to evaluate the inhibition of
Pseudomonas aeruginosa
virulence factors and
Quorum Sensing
during exposure to carvacrol.
P. aeruginosa
(ATCC 10154) was exposed to carvacrol determining changes in biofilm development, motility, acyl-homoserine lactones (AHL) synthesis and relative expression of
lasI/lasR
. Docking analysis was used to determinate interactions between carvacrol with LasI and LasR proteins.
P. aeruginosa
produced 60% lower AHLs when exposed to carvacrol (1.9 mM) compared to control, without affecting cellular viability, indicating a reduction on the LasI synthase activity. AHL-C12, C6, and C4 were detected and related to biofilm development, motility, and pyocyanin production, respectively. The presence of carvacrol reduced the expression of
lasR
, without affecting
lasI
gen. Moreover, computational docking showed interactions of carvacrol with amino acids in the active site pocket of LasI (−5.6 kcal mol
−1
) and within the binding pocket of LasR (−6.7 kcal mol
−1
) of
P. aeruginosa
. These results demonstrated that virulence of
P. aeruginosa
was reduced by carvacrol, by inhibiting LasI activity with the concomitant reduction on the expression of
lasR
, biofilm and swarming motility. This study provides relevant information about the effect of carvacrol against
quorum sensing
to inhibit virulence factors of
P. aeruginosa
at enzymatic and gene levels
.
These findings can contribute to the development of natural anti-QS products, which can affect pathogenesis. |
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ISSN: | 1873-9601 1873-961X |
DOI: | 10.1007/s12079-019-00516-8 |