Multiplexed fluctuation-dissipation-theorem calibration of optical tweezers inside living cells

In order to apply optical tweezers-based force measurements within an uncharacterized viscoelastic medium such as the cytoplasm of a living cell, a quantitative calibration method that may be applied in this complex environment is needed. We describe an improved version of the fluctuation-dissipatio...

Full description

Saved in:
Bibliographic Details
Published in:Review of scientific instruments 2017-11, Vol.88 (11), p.113112-113112
Main Authors: Yan, Hao, Johnston, Jessica F., Cahn, Sidney B., King, Megan C., Mochrie, Simon G. J.
Format: Article
Language:English
Subjects:
Calibration
Cell Physiological Phenomena
Cells (biology)
Cytoplasm
Mechanical Phenomena
Optical Tweezers
Polyethylenes
Saccharomyces - physiology
Scientific apparatus & instruments
Theorems
Variation
Viscoelasticity
Water
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to apply optical tweezers-based force measurements within an uncharacterized viscoelastic medium such as the cytoplasm of a living cell, a quantitative calibration method that may be applied in this complex environment is needed. We describe an improved version of the fluctuation-dissipation-theorem calibration method, which has been developed to perform in situ calibration in viscoelastic media without prior knowledge of the trapped object. Using this calibration procedure, it is possible to extract values of the medium’s viscoelastic moduli as well as the force constant describing the optical trap. To demonstrate our method, we calibrate an optical trap in water, in polyethylene oxide solutions of different concentrations, and inside living fission yeast (S. pombe).
ISSN:0034-6748
1089-7623
DOI:10.1063/1.5012782