Time‐dependent functional, morphological, and molecular changes in diabetic bladder dysfunction in streptozotocin‐induced diabetic mice

Aim Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications of diabetes mellitus (DM). This study aimed to investigate the functional, structural, and molecular changes of the bladder at 0, 3, 6, 9, and 12 weeks after DM induction by streptozotocin (STZ) in male C57...

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Published in:Neurourology and urodynamics 2019-06, Vol.38 (5), p.1266-1277
Main Authors: Yang, Xu‐feng, Wang, Jing, Rui‐Wang, Xu, Yi‐fei, Chen, Fang‐jun, Tang, Li‐yao, Ren, Wen‐kang, Fu, Li‐jun, Tan, Bo, Huang, Ping, Cao, Hong‐ying
Format: Article
Language:English
Subjects:
Animals
Body Weight
compensated state
decompensated state
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Experimental - pathology
diabetic bladder dysfunction (DBD)
Drinking
Electric Stimulation
Glucose Tolerance Test
Male
Mice
Mice, Inbred C57BL
Muscle Contraction - drug effects
Myosin Heavy Chains - biosynthesis
Myosin Heavy Chains - genetics
Myosin Type V - biosynthesis
Myosin Type V - genetics
myosin Va
Nucleotide Transport Proteins - biosynthesis
Nucleotide Transport Proteins - genetics
Original Basic Science
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
SLC17A9
Stimulation, Chemical
temporal changes
Urinary Bladder Diseases - etiology
Urinary Bladder Diseases - genetics
Urinary Bladder Diseases - pathology
Urodynamics
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Summary:Aim Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications of diabetes mellitus (DM). This study aimed to investigate the functional, structural, and molecular changes of the bladder at 0, 3, 6, 9, and 12 weeks after DM induction by streptozotocin (STZ) in male C57BL/6 mice. Methods Male C57BL/6J mice were injected with STZ (130 mg/kg). Then, diabetic general characteristics, cystometry test, histomorphometry, and contractile responses to α, β‐methylene ATP, KCl, electrical‐field stimulation, carbachol were performed at 0, 3, 6, 9, and 12 weeks after induction. Finally, protein and messenger RNA (mRNA) expressions of myosin Va and SLC17A9 were quantified. Results DM mice exhibited lower body weight, voiding efficiency and higher water intake, urine production, fasting blood glucose, oral glucose tolerance test, bladder wall thickness, maximum bladder capacity, residual volume, bladder compliance. In particular, nonvoiding contractions has increased more than five times at 6 weeks. And the amplitudes of spontaneous activity, contractile responses to all stimulus was about two times higher at 6 weeks but cut almost in half at 12 weeks. The protein and mRNA expressions of myosin Va and SLC17A9 were about two times higher at 6 weeks, but myosin Va was reverted nearly 40% while SLC17A9 is still higher at 12 weeks. Conclusions DBD transitioned from a compensated state to a decompensated state in STZ‐induced DM mice at 9 to 12 weeks after DM induction. Our molecular data suggest that the transition may be closely related to the alterations of myosin Va and SLC17A9 expression levels in the bladder with time.
ISSN:0733-2467
1520-6777
1520-6777
DOI:10.1002/nau.24008