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Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water
Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water,...
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Published in: | Scientific reports 2019-11, Vol.9 (1), p.16124-7, Article 16124 |
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description | Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water that has been used to rinse the oral cavity, can be used as an alternative method for collecting saliva for oral microbiome analysis because it can be collected in a shorter time than saliva. The purpose of this study was to verify whether mouth-rinsed water is a suitable saliva substitute for analyzing the oral microbiome. We collected samples of mouth-rinsed water, stimulated saliva, unstimulated saliva, and tongue coating from 10 systemic healthy participants, and compared the microbial diversity and composition of the samples using next-generation sequencing of 16S rRNA-encoding genes. The results showed that the microbial diversity of mouth-rinsed water was similar to that of unstimulated and stimulated saliva, and significantly higher than that of tongue-coating samples. The microbial composition at the species level of mouth-rinsed water also showed a very high correlation with the composition of unstimulated and stimulated saliva. These results suggest that the mouth-rinsed water is a suitable collection method instead of saliva for oral microbiome analysis. |
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However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water that has been used to rinse the oral cavity, can be used as an alternative method for collecting saliva for oral microbiome analysis because it can be collected in a shorter time than saliva. The purpose of this study was to verify whether mouth-rinsed water is a suitable saliva substitute for analyzing the oral microbiome. We collected samples of mouth-rinsed water, stimulated saliva, unstimulated saliva, and tongue coating from 10 systemic healthy participants, and compared the microbial diversity and composition of the samples using next-generation sequencing of 16S rRNA-encoding genes. The results showed that the microbial diversity of mouth-rinsed water was similar to that of unstimulated and stimulated saliva, and significantly higher than that of tongue-coating samples. The microbial composition at the species level of mouth-rinsed water also showed a very high correlation with the composition of unstimulated and stimulated saliva. These results suggest that the mouth-rinsed water is a suitable collection method instead of saliva for oral microbiome analysis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-52445-6</identifier><identifier>PMID: 31695050</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Adult ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Biodiversity ; Cohort Studies ; Epidemiology ; Female ; Fresh Water - microbiology ; Humans ; Male ; Microbiomes ; Microbiota ; Mouth ; Mouth - microbiology ; Mouthwashes - chemistry ; Next-generation sequencing ; Oral cavity ; rRNA 16S ; Saliva ; Saliva - microbiology ; Tongue ; Tongue - microbiology ; Young Adult</subject><ispartof>Scientific reports, 2019-11, Vol.9 (1), p.16124-7, Article 16124</ispartof><rights>2019. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jo, Ryutaro</au><au>Nishimoto, Yuichiro</au><au>Umezawa, Kouta</au><au>Yama, Kazuma</au><au>Aita, Yuto</au><au>Ichiba, Yuko</au><au>Murakami, Shinnosuke</au><au>Kakizawa, Yasushi</au><au>Kumagai, Takashi</au><au>Yamada, Takuji</au><au>Fukuda, Shinji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water</atitle><jtitle>Scientific reports</jtitle><addtitle>Sci Rep</addtitle><date>2019-11-06</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>16124</spage><epage>7</epage><pages>16124-7</pages><artnum>16124</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water that has been used to rinse the oral cavity, can be used as an alternative method for collecting saliva for oral microbiome analysis because it can be collected in a shorter time than saliva. The purpose of this study was to verify whether mouth-rinsed water is a suitable saliva substitute for analyzing the oral microbiome. We collected samples of mouth-rinsed water, stimulated saliva, unstimulated saliva, and tongue coating from 10 systemic healthy participants, and compared the microbial diversity and composition of the samples using next-generation sequencing of 16S rRNA-encoding genes. The results showed that the microbial diversity of mouth-rinsed water was similar to that of unstimulated and stimulated saliva, and significantly higher than that of tongue-coating samples. The microbial composition at the species level of mouth-rinsed water also showed a very high correlation with the composition of unstimulated and stimulated saliva. These results suggest that the mouth-rinsed water is a suitable collection method instead of saliva for oral microbiome analysis.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>31695050</pmid><doi>10.1038/s41598-019-52445-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Cohort Studies Epidemiology Female Fresh Water - microbiology Humans Male Microbiomes Microbiota Mouth Mouth - microbiology Mouthwashes - chemistry Next-generation sequencing Oral cavity rRNA 16S Saliva Saliva - microbiology Tongue Tongue - microbiology Young Adult |
title | Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water |
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