Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water

Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water,...

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Bibliographic Details
Published in:Scientific reports 2019-11, Vol.9 (1), p.16124-7, Article 16124
Main Authors: Jo, Ryutaro, Nishimoto, Yuichiro, Umezawa, Kouta, Yama, Kazuma, Aita, Yuto, Ichiba, Yuko, Murakami, Shinnosuke, Kakizawa, Yasushi, Kumagai, Takashi, Yamada, Takuji, Fukuda, Shinji
Format: Article
Language:English
Subjects:
Adult
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
Cohort Studies
Epidemiology
Female
Fresh Water - microbiology
Humans
Male
Microbiomes
Microbiota
Mouth
Mouth - microbiology
Mouthwashes - chemistry
Next-generation sequencing
Oral cavity
rRNA 16S
Saliva
Saliva - microbiology
Tongue
Tongue - microbiology
Young Adult
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Summary:Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water that has been used to rinse the oral cavity, can be used as an alternative method for collecting saliva for oral microbiome analysis because it can be collected in a shorter time than saliva. The purpose of this study was to verify whether mouth-rinsed water is a suitable saliva substitute for analyzing the oral microbiome. We collected samples of mouth-rinsed water, stimulated saliva, unstimulated saliva, and tongue coating from 10 systemic healthy participants, and compared the microbial diversity and composition of the samples using next-generation sequencing of 16S rRNA-encoding genes. The results showed that the microbial diversity of mouth-rinsed water was similar to that of unstimulated and stimulated saliva, and significantly higher than that of tongue-coating samples. The microbial composition at the species level of mouth-rinsed water also showed a very high correlation with the composition of unstimulated and stimulated saliva. These results suggest that the mouth-rinsed water is a suitable collection method instead of saliva for oral microbiome analysis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-52445-6