Resazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of Mycobacterium abscessus Group

Background Mycobacterium abscessus group has heterogeneous susceptibility pattern among species. The species is most common cause of nosocomial infections. Macrolides minimum Inhibitory concentration (MIC) determination is essential for the treatment. Methods Thirty‐six strains were randomly selecte...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2016-09, Vol.30 (5), p.751-755
Main Authors: Garcia de Carvalho, Natalia Fernandes, Sato, Daisy Nakamura, Pavan, Fernando R., Ferrazoli, Lucilaine, Chimara, Erica
Format: Article
Language:English
Subjects:
Antibiotics
clarithromycin
Clarithromycin - pharmacology
Diagnostic tests
Humans
Microbial Sensitivity Tests - methods
Microbiology
Mycobacterium
Mycobacterium - isolation & purification
Mycobacterium abscessus
Nosocomial infections
Oxazines - metabolism
Pharmacology
resazurin microtiter assay
susceptibility testing
Xanthenes - metabolism
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Summary:Background Mycobacterium abscessus group has heterogeneous susceptibility pattern among species. The species is most common cause of nosocomial infections. Macrolides minimum Inhibitory concentration (MIC) determination is essential for the treatment. Methods Thirty‐six strains were randomly selected for performing Resazurin Microtiter Assay (REMA) for clarithromycin testing in comparison to MIC test according to Clinical and Laboratory Standards Institute (2011) recommendation. REMA has been used for detection of drug resistance in M. tuberculosis. Extended incubation was performed to detect induced resistance. Results Thirty microliters of resazurin (0.01%) was added after visually taking MIC reading. Resistance was observed in 11.1% of M. bolletti and 4.8% of M. abscessus strains; and induced resistance was detected in 77.8% and 95.2% of M. bolletti and M. abscessus strains, respectively. All strains of M. massiliense were susceptible. The samples presented same MIC value both by visual reading and through resazurin. Conclusion The present study showed 100% concordance between both readings, with REMA providing easier to read and report results benefit. This change in reading can also reflect on the MIC determination and report, improving the test.
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.21933