Quantification of ractopamine residues on and in beef digestive tract tissues

Abstract Ractopamine hydrochloride is a commercial beta-adrenergic agonist commonly used as a dietary supplement in cattle production for improved feed efficiency and growth promotion. Currently, regulatory target tissues (as approved in the New Animal Drug Application with Food and Drug Administrat...

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Bibliographic Details
Published in:Journal of animal science 2019-10, Vol.97 (10), p.4193-4198
Main Authors: Davis, Haley E, Badger, Crystal-Dawn, Brophy, Patrick, Geornaras, Ifigenia, Burnett, Thomas J, Scanga, John, Belk, Keith, Prenni, Jessica
Format: Article
Language:English
Subjects:
Abomasum
Adrenergic beta-Agonists - analysis
Animal tissues
Animals
Beef
Beef cattle
Cattle
Cattle - metabolism
Cattle production
Chromatography, Liquid
Contamination
Diet
Digestive system
Digestive tract
Drug Residues - analysis
Feed additives
Feed efficiency
Feeds
Food contamination
Gastrointestinal Tract - metabolism
Intestine
Liver
Liver - metabolism
Mass spectrometry
Meat Science
Metabolism
Muscles
Muscles - metabolism
Phenethylamines - analysis
Quantitation
Ractopamine hydrochloride
Residues
Small intestine
Sympathomimetics
Tandem Mass Spectrometry
Zoology
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Summary:Abstract Ractopamine hydrochloride is a commercial beta-adrenergic agonist commonly used as a dietary supplement in cattle production for improved feed efficiency and growth promotion. Currently, regulatory target tissues (as approved in the New Animal Drug Application with Food and Drug Administration) for ractopamine residue testing are muscle and liver. However, other tissues have recently been subjected to testing in some export markets for U.S. beef, a clear disregard for scientific maximum residue limits associated with specific tissues. The overall goal of this study was to develop and validate an LC-MS/MS assay to determine whether detectable and quantifiable levels of ractopamine in digestive tract-derived edible offal items (i.e., abomasum, omasum, small intestine, and reticulum) of cattle resulted from tissue residues or residual ingesta contamination of exposed surfaces of tissues (rinsates). Tissue samples and corresponding rinsates from 10 animals were analyzed for parent and total ractopamine (tissue samples only). The lower limit of quantitation was between 0.03 and 0.66 ppb depending on the tissue type, and all tissue and rinsate samples tested had quantifiable concentrations of ractopamine. The highest concentrations of tissue-specific ractopamine metabolism (represented by higher total vs. parent ractopamine levels) were observed in liver and small intestine. Contamination from residual ingesta (represented by detectable ractopamine in rinsate samples) was only detected in small intestine, with a measured mean concentration of 19.72 ppb (±12.24 ppb). Taken together, these results underscore the importance of the production process and suggest that improvements may be needed to reduce the likelihood of contamination from residual ractopamine in digestive tract-derived edible offal tissues for market.
ISSN:0021-8812
1525-3163
DOI:10.1093/jas/skz263