Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model

ABSTRACT The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post‐traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral...

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Bibliographic Details
Published in:Journal of orthopaedic research 2019-05, Vol.37 (5), p.1071-1079
Main Authors: Feeney, Elizabeth, Peal, Bridgette T., Inglis, Jacqueline E., Su, Jin, Nixon, Alan J., Bonassar, Lawrence J., Reesink, Heidi L.
Format: Article
Language:English
Subjects:
Animals
articular fracture
Carpal Joints - injuries
Carpal Joints - metabolism
Disease Models, Animal
Female
Glycoproteins - metabolism
Horses
hyaluronic acid (HA)
Hyaluronic Acid - metabolism
lubricin/PRG4
Male
Osteoarthritis - etiology
Osteoarthritis - metabolism
post‐traumatic osteoarthritis
Synovial Fluid - metabolism
viscosity
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Summary:ABSTRACT The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post‐traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham‐operated control. Horses were exercised on a high‐speed treadmill, and synovial fluid was collected pre‐operatively and at serial timepoints until 75 days post‐operatively. Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis. Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively. HA concentrations were significantly decreased post‐operatively, and high molecular weight HA (>6.1MDa) did not recover to pre‐operative values by the study termination at day 75. Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham‐operated limbs. Viscosity was significantly reduced after surgery. While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower. Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham‐operated limbs, which may be explained by synovial fluid washout during arthroscopy. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.24281