Nucleic Acid‐Barcoding Technologies: Converting DNA Sequencing into a Broad‐Spectrum Molecular Counter

The emergence of high‐throughput DNA sequencing technologies sparked a revolution in the field of genomics that has rippled into many branches of the life and physical sciences. The remarkable sensitivity, specificity, throughput, and multiplexing capacity that are inherent to parallel DNA sequencin...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2019-03, Vol.58 (13), p.4144-4162
Main Authors: Liszczak, Glen, Muir, Tom W.
Format: Article
Language:English
Subjects:
Bar codes
chemical biology
Deoxyribonucleic acid
DNA
DNA sequencing
drug discovery
high-throughput biochemistry
Multiplexing
Nucleic acids
nucleic-acid chemistry
Nucleotide sequence
Organic chemistry
Physical sciences
protein chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The emergence of high‐throughput DNA sequencing technologies sparked a revolution in the field of genomics that has rippled into many branches of the life and physical sciences. The remarkable sensitivity, specificity, throughput, and multiplexing capacity that are inherent to parallel DNA sequencing have since motivated its use as a broad‐spectrum molecular counter. A key aspect of extrapolating DNA sequencing to non‐traditional applications is the need to append nucleic‐acid barcodes to entities of interest. In this review, we describe the chemical and biochemical approaches that have enabled nucleic‐acid barcoding of proteinaceous and non‐proteinaceous materials and provide examples of downstream technologies that have been made possible by DNA‐encoded molecules. As commercially available high‐throughput sequencers were first released less than 15 years ago, we believe related applications will continue to mature and close by proposing new frontiers to support this assertion. Parallel DNA sequencing offers remarkable detection sensitivity, specificity, and throughput capacity that have motivated its use as a broad‐spectrum molecular counter across many fields of science. Herein, we describe the chemical and biochemical approaches that have been developed to append DNA barcodes to a range of biological and non‐biological materials and highlight technologies that have been made possible by DNA‐encoded molecules.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201808956