On-off switching of cell cycle and melanogenesis regulation of melanocytes by non-thermal atmospheric pressure plasma-activated medium

Non-thermal atmospheric pressure (NAP) plasma has demonstrated potential in biomedical applications, such as cancer treatment, bactericidal sterilization, and cell growth promotion or inhibition. In this study, for the first time, we demonstrated on-off switching of cell cycle progression and regula...

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Bibliographic Details
Published in:Scientific reports 2019-09, Vol.9 (1), p.13400-10, Article 13400
Main Authors: Lee, Jin-Woo, Han, Se Jik, Kang, Hye Young, Wi, Sung-Suk, Jung, Min-Hyung, Kim, Kyung Sook
Format: Article
Language:English
Subjects:
Atmospheric Pressure
Cell Cycle
Cell growth
Cell proliferation
Cell Survival
Cells, Cultured
Cytotoxicity
Dopachrome isomerase
Humans
Melanins - metabolism
Melanocytes
Melanocytes - cytology
Melanocytes - drug effects
Plasma Gases - pharmacology
Reactive oxygen species
Reactive Oxygen Species - metabolism
Skin
Skin - cytology
Skin - drug effects
Sterilization
Temperature
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Summary:Non-thermal atmospheric pressure (NAP) plasma has demonstrated potential in biomedical applications, such as cancer treatment, bactericidal sterilization, and cell growth promotion or inhibition. In this study, for the first time, we demonstrated on-off switching of cell cycle progression and regulated melanogenesis in normal human skin melanocytes by NAP plasma-activated medium (PAM). The melanocytes were exposed to NAP plasma at durations varying from 0 to 20 min, and the effects of PAM on cell proliferation, cell cycle progression, and melanogenesis were investigated. Although PAM showed no cytotoxicity, the proliferation of melanocytes was inhibited. The melanocyte cell cycle was arrested by PAM for a relatively short period (48 h), after which it recovered slowly. PAM promoted melanogenesis through the activation of the enzymes tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. These effects seem to be related to reactive oxygen species induced by PAM. Our finding that PAM modulates the cell cycle may provide insight into the recurrence of cancer. The regulation of the melanogenesis of melanocytes may facilitate the control of skin tone without incurring negative side effects.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-50041-2