Sequence-Specific Detection of Unlabeled Nucleic Acid Biomarkers Using a “One-Pot” 3D Molecular Sensor

DNA and RNA biomarkers have not progressed beyond the automated specialized clinic due to failure in the reproducibility necessary to standardize robust and rapid nucleic acid detection at the point of care, where health outcomes can be most improved by early-stage diagnosis and precise monitoring o...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-08, Vol.91 (15), p.10016-10025
Main Authors: Yousaf, Sameen, King, Patrick J. S, Miller, Aline F, Saiani, Alberto, Clarke, David J, Trivoluzzi, Linda T, Aojula, Harmesh S, Bichenkova, Elena V
Format: Article
Language:English
Subjects:
Abnormalities
Base Pair Mismatch
Base Sequence
Biomarkers
Biomaterials
Biomedical materials
Chemistry
Deoxyribonucleic acid
Detection limits
DNA
Fluorescence
Genetic abnormalities
Hydrogels
Hydrogels - chemistry
Limit of Detection
Mass spectrometry
Mass spectroscopy
Mutation
NMR
Nuclear magnetic resonance
Nucleic Acid Hybridization
Nucleic Acids - analysis
Nucleotides
Oligonucleotides
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Oligonucleotides - metabolism
Peptides
Peptides - chemical synthesis
Peptides - chemistry
Peptides - metabolism
Polymerase Chain Reaction - methods
Protein structure
Rheological properties
Rheology
Ribonucleic acid
RNA
Secondary structure
Single-nucleotide polymorphism
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Summary:DNA and RNA biomarkers have not progressed beyond the automated specialized clinic due to failure in the reproducibility necessary to standardize robust and rapid nucleic acid detection at the point of care, where health outcomes can be most improved by early-stage diagnosis and precise monitoring of therapy and disease prognosis. We demonstrate here a new analytical platform to meet this challenge using functional 3D hydrogels engineered from peptide and oligonucleotide building blocks to provide sequence-specific, PCR-free fluorescent detection of unlabeled nucleic acid sequences. We discriminated at picomolar detection limits (
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b01841