CD93 is a cell surface lectin receptor involved in the control of the inflammatory response stimulated by exogenous DNA

Summary Bacterial DNA contains CpG oligonucleotide (ODN) motifs to trigger innate immune responses through the endosomal receptor Toll‐like receptor 9 (TLR9). One of the cell surface receptors to capture and deliver microbial DNA to intracellular TLR9 is the C‐type lectin molecule DEC‐205 through it...

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Bibliographic Details
Published in:Immunology 2019-10, Vol.158 (2), p.85-93
Main Authors: Nativel, Brice, Ramin‐Mangata, Stéphane, Mevizou, Rudy, Figuester, Audrey, Andries, Jessica, Iwema, Thomas, Ikewaki, Nobunao, Gasque, Philippe, Viranaïcken, Wildriss
Format: Article
Language:English
Subjects:
Antigens, CD - genetics
Antigens, CD - immunology
Bacteria
bacterial DNA
Binding
Biological Transport - genetics
Biological Transport - immunology
CD93
Cell Line, Tumor
Cell surface
Cloning, Molecular
CpG islands
CpG oligonucleotide
C‐type lectin‐like domain
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
DNA, Bacterial - immunology
DNA, Bacterial - metabolism
Endosomes - immunology
Endosomes - metabolism
Enzyme-linked immunosorbent assay
Escherichia coli - chemistry
Escherichia coli - genetics
Escherichia coli - metabolism
Fluorescence
Gene Expression
Gene Expression Regulation - immunology
Genetic Vectors - chemistry
Genetic Vectors - metabolism
Histidine
Humans
Immune response
Inflammation
Inflammatory response
Innate immunity
Interleukin-6 - genetics
Interleukin-6 - immunology
Interleukins
Lectins
Lectins, C-Type - genetics
Lectins, C-Type - immunology
Membrane Glycoproteins - genetics
Membrane Glycoproteins - immunology
Membrane Glycoproteins - metabolism
Microorganisms
Minor Histocompatibility Antigens - genetics
Minor Histocompatibility Antigens - immunology
Models, Biological
Neuroblastoma cells
Neurons - immunology
Neurons - metabolism
Neurons - pathology
Oligodeoxyribonucleotides - genetics
Oligodeoxyribonucleotides - immunology
Oligodeoxyribonucleotides - metabolism
Oligonucleotides
Original
Protein Binding
Protein Domains
Receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - immunology
Receptors, Complement - genetics
Receptors, Complement - immunology
Receptors, Complement - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - metabolism
Signal Transduction
TLR9 protein
Toll-Like Receptor 9 - genetics
Toll-Like Receptor 9 - immunology
Toll-like receptors
Toll‐like receptor 9
Tryptophan
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Summary:Summary Bacterial DNA contains CpG oligonucleotide (ODN) motifs to trigger innate immune responses through the endosomal receptor Toll‐like receptor 9 (TLR9). One of the cell surface receptors to capture and deliver microbial DNA to intracellular TLR9 is the C‐type lectin molecule DEC‐205 through its N‐terminal C‐type lectin‐like domain (CTLD). CD93 is a cell surface protein and member of the lectin group XIV with a CTLD. We hypothesized that CD93 could interact with CpG motifs, and possibly serve as a novel receptor to deliver bacterial DNA to endosomal TLR9. Using ELISA and tryptophan fluorescence binding studies we observed that the soluble histidine‐tagged CD93‐CTLD was specifically binding to CpG ODN and bacterial DNA. Moreover, we found that CpG ODN could bind to CD93‐expressing IMR32 neuroblastoma cells and induced more robust interleukin‐6 secretion when compared with mock‐transfected IMR32 control cells. Our data argue for a possible contribution of CD93 to control cell responsiveness to bacterial DNA in a manner reminiscent of DEC‐205. We postulate that CD93 may act as a receptor at plasma membrane for DNA or CpG ODN and to grant delivery to endosomal TLR9. The recognition of exogenous DNA leads to an inflammatory response. The exogenous DNA bind to CD93 and it deliver to endosomal TLR9. This process allows the production of proinflammatory cytokines mainly mediated by NF‐κB/AP1 pathway.
ISSN:0019-2805
1365-2567
DOI:10.1111/imm.13100