Lovastatin attenuates hypertension induced by renal tubule‐specific knockout of ATP‐binding cassette transporter A1, by inhibiting epithelial sodium channels

Background and Purpose We have shown that cholesterol is synthesized in the principal cells of renal cortical collecting ducts (CCD) and stimulates the epithelial sodium channels (ENaC). Here we have determined whether lovastatin, a cholesterol synthesis inhibitor, can antagonize the hypertension in...

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Published in:British journal of pharmacology 2019-09, Vol.176 (18), p.3695-3711
Main Authors: Wu, Ming‐Ming, Liang, Chen, Yu, Xiao‐Di, Song, Bin‐Lin, Yue, Qiang, Zhai, Yu‐Jia, Linck, Valerie, Cai, Yong‐Xu, Niu, Na, Yang, Xu, Zhang, Bao‐Long, Wang, Qiu‐Shi, Zou, Li, Zhang, Shuai, Thai, Tiffany L., Ma, Jing, Sutliff, Roy L., Zhang, Zhi‐Ren, Ma, He‐Ping
Format: Article
Language:English
Subjects:
ABCA1 protein
Animals
Anticholesteremic Agents - pharmacology
Antihypertensive Agents - pharmacology
Antihypertensive Agents - therapeutic use
ATP
ATP Binding Cassette Transporter 1 - genetics
ATP-binding protein
Channel gating
Cholesterol
Clonal deletion
Cyclosporins
Epithelial Sodium Channel Blockers - pharmacology
Epithelial Sodium Channel Blockers - therapeutic use
Epithelial Sodium Channels - physiology
Furin
Glucocorticoids
Hypertension
Hypertension - drug therapy
Hypertension - metabolism
Hypertension - physiopathology
Immunofluorescence
Kidney Tubules - metabolism
Kinases
Lovastatin
Lovastatin - pharmacology
Lovastatin - therapeutic use
Male
Mice, Knockout
Oxidative stress
Reactive oxygen species
Research Paper
Research Papers
Sodium
Sodium channels
Statins
Urine
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Summary:Background and Purpose We have shown that cholesterol is synthesized in the principal cells of renal cortical collecting ducts (CCD) and stimulates the epithelial sodium channels (ENaC). Here we have determined whether lovastatin, a cholesterol synthesis inhibitor, can antagonize the hypertension induced by activated ENaC, following deletion of the cholesterol transporter (ATP‐binding cassette transporter A1; ABCA1). Experimental Approach We selectively deleted ABCA1 in the principal cells of mouse CCD and used the cell‐attached patch‐clamp technique to record ENaC activity. Western blot and immunofluorescence staining were used to evaluate protein expression levels. Systolic BP was measured with the tail‐cuff method. Key Results Specific deletion of ABCA1 elevated BP and ENaC single‐channel activity in the principal cells of CCD in mice. These effects were antagonized by lovastatin. ABCA1 deletion elevated intracellular cholesterol levels, which was accompanied by elevated ROS, increased expression of serum/glucocorticoid regulated kinase 1 (Sgk1), phosphorylated neural precursor cell‐expressed developmentally down‐regulated protein 4‐2 (Nedd4‐2) and furin, along with shorten the primary cilium, and reduced ATP levels in urine. Conclusions and Implications These data suggest that specific deletion of ABCA1 in principal cells increases BP by stimulating ENaC channels via a cholesterol‐dependent pathway which induces several secondary responses associated with oxidative stress, activated Sgk1/Nedd4‐2, increased furin expression, and reduced cilium‐mediated release of ATP. As ABCA1 can be blocked by cyclosporine A, these results suggest further investigation of the possible use of statins to treat CsA‐induced hypertension.
ISSN:0007-1188
1476-5381
DOI:10.1111/bph.14775