Revisiting the genetic diversity of emerging hantaviruses circulating in Europe using a pan-viral resequencing microarray

Hantaviruses are zoonotic agents transmitted from small mammals, mainly rodents, to humans, where they provoke diseases such as Hemorrhagic fever with Renal Syndrome (HFRS) and its mild form, Nephropathia Epidemica (NE), or Hantavirus Cardio-Pulmonary Syndrome (HCPS). Hantaviruses are spread worldwi...

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Bibliographic Details
Published in:	Scientific reports 2019-08, Vol.9 (1), p.12404-13, Article 12404
Main Authors:	Filippone, Claudia, Castel, Guillaume, Murri, Séverine, Ermonval, Myriam, Korva, Misa, Avšič-Županc, Tatjana, Sironen, Tarja, Vapalahati, Olli, McElhinney, Lorraine M, Ulrich, Rainer G, Groschup, Martin H, Caro, Valérie, Sauvage, Frank, van der Werf, Sylvie, Manuguerra, Jean-Claude, Gessain, Antoine, Marianneau, Philippe, Tordo, Noël
Format:	Article
Language:	eng
Subjects:	Europe Fever Genetic diversity Genetic Variation Hantavirus - classification Hantavirus - genetics Hantavirus - isolation & purification Hantavirus Infections - pathology Hantavirus pulmonary syndrome Hemorrhage Hemorrhagic fever with renal syndrome Humans Life Sciences Microbiology and Parasitology Oligonucleotide Array Sequence Analysis - methods Phylogeny Phylogeography Puumala virus - classification Puumala virus - genetics RNA, Viral - genetics RNA, Viral - metabolism Virology Zoonoses
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Summary:	Hantaviruses are zoonotic agents transmitted from small mammals, mainly rodents, to humans, where they provoke diseases such as Hemorrhagic fever with Renal Syndrome (HFRS) and its mild form, Nephropathia Epidemica (NE), or Hantavirus Cardio-Pulmonary Syndrome (HCPS). Hantaviruses are spread worldwide and monitoring animal reservoirs is of primary importance to control the zoonotic risk. Here, we describe the development of a pan-viral resequencing microarray (PathogenID v3.0) able to explore the genetic diversity of rodent-borne hantaviruses endemic in Europe. Among about 800 sequences tiled on the microarray, 52 correspond to a tight molecular sieve of hantavirus probes covering a large genetic landscape. RNAs from infected animal tissues or from laboratory strains have been reverse transcribed, amplified, then hybridized to the microarray. A classical BLASTN analysis applied to the sequence delivered through the microarray allows to identify the hantavirus species up to the exact geographical variant present in the tested samples. Geographical variants of the most common European hantaviruses from France, Germany, Slovenia and Finland, such as Puumala virus, Dobrava virus and Tula virus, were genetically discriminated. Furthermore, we precisely characterized geographical variants still unknown when the chip was conceived, such as Seoul virus isolates, recently emerged in France and the United Kingdom.
ISSN:	2045-2322 2045-2322