Compartmentalization of the GABAB receptor signaling complex is required for presynaptic inhibition at hippocampal synapses

Presynaptic inhibition via G-protein-coupled receptors (GPCRs) and voltage-gated Ca(2+) channels constitutes a widespread regulatory mechanism of synaptic strength. Yet, the mechanism of intermolecular coupling underlying GPCR-mediated signaling at central synapses remains unresolved. Using FRET spe...

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Bibliographic Details
Published in:The Journal of neuroscience 2011-08, Vol.31 (35), p.12523-12532
Main Authors: Laviv, Tal, Vertkin, Irena, Berdichevsky, Yevgeny, Fogel, Hilla, Riven, Inbal, Bettler, Bernhard, Slesinger, Paul A, Slutsky, Inna
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Baclofen - pharmacology
Calcium - metabolism
Calcium Channels, N-Type - genetics
Calcium Channels, N-Type - metabolism
Cyclic AMP - metabolism
Electric Stimulation
GABA Antagonists - pharmacology
GABA-B Receptor Agonists - pharmacology
GTP-Binding Protein beta Subunits - genetics
GTP-Binding Protein beta Subunits - metabolism
GTP-Binding Protein gamma Subunits - genetics
GTP-Binding Protein gamma Subunits - metabolism
Hippocampus - cytology
Luminescent Proteins - genetics
Mice
Mice, Inbred BALB C
Mice, Knockout
Microscopy, Confocal
Mutation - genetics
Neural Inhibition - drug effects
Neural Inhibition - physiology
Organophosphorus Compounds - pharmacology
Pertussis Toxin - pharmacology
Picrotoxin - pharmacology
Presynaptic Terminals - drug effects
Presynaptic Terminals - physiology
Rats
Rats, Wistar
Receptors, GABA-B - deficiency
Receptors, GABA-B - metabolism
Signal Transduction - genetics
Signal Transduction - physiology
Spectroscopy, Fourier Transform Infrared - methods
Synapses - drug effects
Synapses - physiology
Synaptic Vesicles - metabolism
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Description
Summary:Presynaptic inhibition via G-protein-coupled receptors (GPCRs) and voltage-gated Ca(2+) channels constitutes a widespread regulatory mechanism of synaptic strength. Yet, the mechanism of intermolecular coupling underlying GPCR-mediated signaling at central synapses remains unresolved. Using FRET spectroscopy, we provide evidence for formation of spatially restricted (
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.1527-11.2011