Synergistic Antimicrobial Activity by Light or Thermal Treatment and Lauric Arginate: Membrane Damage and Oxidative Stress

The need for more effective antimicrobials is critical for the food industry to improve food safety and reduce spoilage of minimally processed foods. The present study was initiated to develop an efficient and novel antimicrobial approach which combines physical treatments (UV-A or mild heat) and ge...

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Bibliographic Details
Published in:Applied and environmental microbiology 2019-09, Vol.85 (17)
Main Authors: Yang, Xu, Rai, Rewa, Huu, Cuong Nguyen, Nitin, Nitin
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antioxidants
Bacteria
Cell membranes
Damage
Deactivation
Dietary supplements
Disruption
E coli
Food industry
Food Microbiology
Food processing
Food processing industry
Food safety
Food spoilage
Heat
Heat treatment
Inactivation
Liposomes
Listeria
Listeria innocua
Minimum inhibitory concentration
Mode of action
Oxidative stress
Polymyxin B
Spoilage
Ultraviolet radiation
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Summary:The need for more effective antimicrobials is critical for the food industry to improve food safety and reduce spoilage of minimally processed foods. The present study was initiated to develop an efficient and novel antimicrobial approach which combines physical treatments (UV-A or mild heat) and generally recognized as safe lauroyl arginate ethyl (LAE) to inactivate surrogate strains, including and Synergistic inactivation of bacteria resulted in an ∼6-log reduction of target bacteria, while individual treatments resulted in 5-log reduction. Further mechanistic study revealed that oxidative stress is responsible for synergistic inactivation between LAE and UV-A, while both membrane damage and oxidative stress are responsible for the synergistic combination between LAE and mild heat. The mode of action of LAE was further compared to that of polymyxin B and analyzed using artificial membrane model systems and the addition of antioxidants. The proposed combinati
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.01033-19