Integrative analysis of the intestinal metabolome of childhood asthma

The intestinal metabolome reflects the biological consequences of diverse exposures and might provide insight into asthma pathophysiology. We sought to perform an untargeted integrative analysis of the intestinal metabolome of childhood asthma in this ancillary study of the Vitamin D Antenatal Asthm...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2019-08, Vol.144 (2), p.442-454
Main Authors: Lee-Sarwar, Kathleen A., Kelly, Rachel S., Lasky-Su, Jessica, Zeiger, Robert S., O'Connor, George T., Sandel, Megan T., Bacharier, Leonard B., Beigelman, Avraham, Laranjo, Nancy, Gold, Diane R., Weiss, Scott T., Litonjua, Augusto A.
Format: Article
Language:English
Subjects:
Age
Antibiotics
Asthma
Asthma - metabolism
Asthma - microbiology
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
Breast
breast-feeding
Breastfeeding & lactation
Child, Preschool
Children
Children & youth
Christensenellaceae
Correlation analysis
Diet
Eigenvalues
Feces - microbiology
Female
Gastrointestinal Microbiome
Humans
Infant
Infant, Newborn
Intestine
Lipid metabolism
Lipids
Male
Mass spectrometry
Mass spectroscopy
Metabolites
Metabolome
Metabolomics
microbiome
Microbiomes
nutrition
Parent educational background
Polyunsaturated fatty acids
Precision medicine
Principal components analysis
Questionnaires
RNA, Bacterial - genetics
RNA, Bacterial - metabolism
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 16S - metabolism
rRNA 16S
Vitamin D
vitamin E
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Summary:The intestinal metabolome reflects the biological consequences of diverse exposures and might provide insight into asthma pathophysiology. We sought to perform an untargeted integrative analysis of the intestinal metabolome of childhood asthma in this ancillary study of the Vitamin D Antenatal Asthma Reduction Trial. Metabolomic profiling was performed by using mass spectrometry on fecal samples collected from 361 three-year-old subjects. Adjusted logistic regression analyses identified metabolites and modules of highly correlated metabolites associated with asthma diagnosis by age 3 years. Sparse canonical correlation analysis identified associations relevant to asthma between the intestinal metabolome and other “omics”: the intestinal microbiome as measured by using 16S rRNA sequencing, the plasma metabolome as measured by using mass spectrometry, and diet as measured by using food frequency questionnaires. Several intestinal metabolites were associated with asthma at age 3 years, including inverse associations between asthma and polyunsaturated fatty acids (adjusted logistic regression β = −6.3; 95% CI, −11.3 to −1.4; P = .01) and other lipids. Asthma-associated intestinal metabolites were significant mediators of the inverse relationship between exclusive breast-feeding for the first 4 months of life and asthma (P for indirect association = .04) and the positive association between a diet rich in meats and asthma (P = .03). Specific intestinal bacterial taxa, including the family Christensenellaceae, and plasma metabolites, including γ-tocopherol/β-tocopherol, were positively associated with asthma and asthma-associated intestinal metabolites. Integrative analyses revealed significant interrelationships between the intestinal metabolome and the intestinal microbiome, plasma metabolome, and diet in association with childhood asthma. These findings require replication in future studies. [Display omitted]
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2019.02.032