Binding of anti-HLA class I antibody to endothelial cells produce an inflammatory cytokine secretory pattern

Current methods are inadequate for the diagnosis of early chronic allograft rejection. The goal of this study was to determine whether ligation of anti‐HLA antibodies to endothelial cells is associated with a distinctive cytokine secretory pattern. Human iliac artery endothelial cells (HIAEC) cultur...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2009, Vol.23 (3), p.157-160
Main Authors: Reyes-Vargas, Eduardo, Pavlov, Igor Y., Martins, Thomas B., Schwartz, Jason J., Hill, Harry R., Delgado, Julio C.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - pharmacology
Arteries
Cell culture
Cell Proliferation - drug effects
Cells, Cultured
cytokines
Cytokines - secretion
Endothelial cells
Endothelial Cells - drug effects
Endothelial Cells - immunology
Endothelial Cells - metabolism
Graft rejection
Graft Rejection - immunology
Histocompatibility antigen HLA
Histocompatibility Antigens Class I - immunology
HLA antibody
Humans
Iliac Artery - cytology
Immunoassays
Inflammation
Interleukin 6
Interleukin 8
Interleukins - metabolism
microspheres
Monoclonal antibodies
Original
Transplantation, Homologous - immunology
Tumor Necrosis Factor-alpha - metabolism
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Summary:Current methods are inadequate for the diagnosis of early chronic allograft rejection. The goal of this study was to determine whether ligation of anti‐HLA antibodies to endothelial cells is associated with a distinctive cytokine secretory pattern. Human iliac artery endothelial cells (HIAEC) cultured in vitro were incubated with w6/32, an anti‐HLA class I mAb. Culture supernatants collected daily for up to 4 days were tested for secretion of 13 cytokines using a multiplexed fluorescent microsphere immunoassay. Culture of HIAEC with medium containing mAb w6/32 supported the growth of HIAEC during the 4‐day study period. Levels of the pro‐inflammatory cytokines IL‐1β, IL‐6, IL‐8, and TNF‐α became significantly increased in supernatants of HIAEC incubated with the mAb w6/32. We conclude that ligation of anti‐HLA class I antibodies to HLA class I antigens in endothelial cells initiates an acute inflammatory process and detecting an inflammatory cytokine secretory pattern might be useful to diagnose sub‐clinical chronic allograft rejection. J. Clin. Lab. Anal. 23:157–160, 2009. © 2009 Wiley‐Liss, Inc.
ISSN:0887-8013
1098-2825
1098-2825
DOI:10.1002/jcla.20310