Lipid peroxidation and antioxidant enzyme activities in cancerous bladder tissue and their relation with bacterial infection: a controlled clinical study

It is well known that antioxidants and reactive oxygen species play an important role in carcinogenesis. In this sudy, we attempted to evaluate antioxidant enzyme activities and lipid peroxidation levels in cancerous bladder tissue and to determine their relationship with bacterial infection. Bacter...

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Published in:Journal of clinical laboratory analysis 2010, Vol.24 (1), p.25-30
Main Authors: Bayraktar, Nihayet, Kilic, Suleyman, Bayraktar, Mehmet Refik, Aksoy, Nurten
Format: Article
Language:English
Subjects:
Adult
Agar
Aged
Aged, 80 and over
Antioxidants
Antioxidants - metabolism
Bacteria
Bacterial Infections - metabolism
Bacterial Infections - urine
bladder cancer
Blood
Carcinogenesis
Case-Control Studies
Catalase
Catalase - metabolism
Enzymes
Female
Free radicals
Glutathione peroxidase
Glutathione Peroxidase - metabolism
Humans
Indexing in process
Infection
Lipid Peroxidation
Male
Methylene blue
Middle Aged
Original
Oxidative stress
Reactive oxygen species
Statistics, Nonparametric
Superoxide dismutase
Superoxide Dismutase - metabolism
thiobarbituric acid
thiobarbituric acid reactive substances
Thiobarbituric Acid Reactive Substances - metabolism
Tumors
Urinary bladder
Urinary Bladder Neoplasms - enzymology
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - microbiology
Urinary Bladder Neoplasms - urine
Urinary Tract Infections - metabolism
Urinary Tract Infections - urine
Urine
Xanthine oxidase
Xanthine Oxidase - metabolism
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Summary:It is well known that antioxidants and reactive oxygen species play an important role in carcinogenesis. In this sudy, we attempted to evaluate antioxidant enzyme activities and lipid peroxidation levels in cancerous bladder tissue and to determine their relationship with bacterial infection. Bacterial culture was made from all urine samples using Blood and Eosin Methylene Blue agars for checking the presence of bacterial infections. We measured thiobarbituric acid reactive substances (TBARs) and activities of xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSH‐PX), and catalase (CAT) in cancerous tissues of 25 bladder cancer patients, in noncancerous adjacent bladder tissues of 13 out of these 25 patients, and in control bladder tissues of 15 patients with a non‐neoplastic genitourinary disease. TBARs levels increased and XO, SOD, GSH‐PX, and CAT activities decreased significantly in cancerous bladder tissues. TBARS, XO, and SOD levels were not significantly different between noncancerous adjacent tissue and control bladder tissue. Statistically significantly lower GSH‐PX and higher CAT activities were observed in noncancerous adjacent bladder tissue compared with cancerous tissue. GSH‐PX level of tumor tissue was correlated significantly with tumor grade (r=−0.425, P=0.034). Results suggested that pathway activity of free radicals were accelerated in the cancerous human bladder tissues via increased TBARs levels and decreased enzyme activities of XO, SOD, GSH‐PX, and CAT, which implicated a severe exposure of cancerous tissues to oxidative stress. J. Clin. Lab. Anal. 24:25–30, 2010. © 2010 Wiley‐Liss, Inc.
ISSN:0887-8013
1098-2825
1098-2825
DOI:10.1002/jcla.20356