Genome‐wide functional characterization of putative peroxidases in the head blight fungus Fusarium graminearum

Summary Reactive oxygen species (ROS) are associated with various developmental processes and host–pathogen interactions in pathogenic fungi. Peroxidases are a group of ROS‐detoxifying enzymes that are involved in the oxidative stress response and in a variety of physiological processes. In this stu...

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Bibliographic Details
Published in:Molecular plant pathology 2018-03, Vol.19 (3), p.715-730
Main Authors: Lee, Yoonji, Son, Hokyoung, Shin, Ji Young, Choi, Gyung Ja, Lee, Yin‐Won
Format: Article
Language:English
Subjects:
Antioxidants
Blight
Cereal crops
Deletion mutant
Fungi
Fusarium
Fusarium graminearum
Genes
Genomes
Host plants
Host-pathogen interactions
Hydrogen peroxide
Mutants
Original
Oxidative stress
oxidative stress response
Pathogens
Peroxidase
Phenotypes
Reactive oxygen species
Regulators
Transcription factors
Virulence
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Summary:Summary Reactive oxygen species (ROS) are associated with various developmental processes and host–pathogen interactions in pathogenic fungi. Peroxidases are a group of ROS‐detoxifying enzymes that are involved in the oxidative stress response and in a variety of physiological processes. In this study, we performed a genome‐wide functional characterization of putative peroxidase genes in Fusarium graminearum, a head blight pathogen of cereal crops. We identified 31 putative peroxidase genes and generated deletion mutants for these genes. Twenty‐six of the deletion mutants showed developmental phenotypes indistinguishable from that of the wild‐type, and five deletion mutants exhibited phenotypic changes in at least one phenotypic category. Four deletion mutants, fca6, fca7, fpx1 and fpx15, showed increased sensitivity to extracellular H2O2. Deletion mutants of FCA7 also exhibited reduced virulence and increased trichothecene production compared with those of the wild‐type strain, suggesting that Fca7 may play an important role in the host–pathogen interaction in F. graminearum. To identify the transcription factors (TFs) regulating FCA6, FCA7, FPX1 and FPX15 in response to oxidative stress, we screened an F. graminearum TF mutant library for growth in the presence of H2O2 and found that multiple TFs co‐regulated the expression of FCA7 under oxidative stress conditions. These results demonstrate that a complex network of transcriptional regulators of antioxidant genes is involved in oxidative stress responses in this fungus. Moreover, our study provides insights into the roles of peroxidases in developmental processes and host–pathogen interactions in plant‐pathogenic fungi.
ISSN:1464-6722
1364-3703
DOI:10.1111/mpp.12557