Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex of Neurospora crassa

is an established reference organism to investigate carotene biosynthesis and light regulation. However, there is little evidence of its capacity to produce secondary metabolites. Here, we report the role of the fungal-specific regulatory velvet complexes in development and secondary metabolism (SM)...

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Published in:	Genetics (Austin) 2019-07, Vol.212 (3), p.691-710
Main Authors:	Bayram, Özlem Sarikaya, Dettmann, Anne, Karahoda, Betim, Moloney, Nicola M, Ormsby, Tereza, McGowan, Jamie, Cea-Sánchez, Sara, Miralles-Durán, Alejandro, Brancini, Guilherme T P, Luque, Eva M, Fitzpatrick, David A, Cánovas, David, Corrochano, Luis M, Doyle, Sean, Selker, Eric U, Seiler, Stephan, Bayram, Özgür
Format:	Article
Language:	eng
Subjects:	Active Transport, Cell Nucleus Biosynthesis Carotene Carotenoids - metabolism Cell Nucleus - metabolism Complementation Complex formation DNA methylation Functionals Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Gene expression Gene Expression Regulation, Fungal Genomes Investigations Iron Iron and steel making Kinases Light Localization Metabolism Metabolites Methyltransferase Methyltransferases - genetics Methyltransferases - metabolism Mutants Neurospora Neurospora crassa Neurospora crassa - genetics Neurospora crassa - metabolism Neurospora crassa - physiology Neurospora crassa - radiation effects Niches Ontogeny Phenotypes Phosphorylation Protein Multimerization Proteins Secondary metabolites Signal transduction Spores, Fungal - genetics Spores, Fungal - physiology Sporulation Starvation Transcription Factors - genetics Transcription Factors - metabolism
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Summary:	is an established reference organism to investigate carotene biosynthesis and light regulation. However, there is little evidence of its capacity to produce secondary metabolites. Here, we report the role of the fungal-specific regulatory velvet complexes in development and secondary metabolism (SM) in Three velvet proteins VE-1, VE-2, VOS-1, and a putative methyltransferase LAE-1 show light-independent nucleocytoplasmic localization. Two distinct velvet complexes, a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 are found The heterotrimer-complex, which positively regulates sexual development and represses asexual sporulation, suppresses siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controls carotene production. VE-1 regulates the expression of >15% of the whole genome, comprising mainly regulatory and developmental features. We also studied intergenera functions of the velvet complex through complementation of , , , mutants with their orthologs , , , and , respectively. Expression of VE-1 and VE-2 in successfully substitutes the developmental and SM functions of VeA and VelB by forming two functional chimeric velvet complexes , VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. Reciprocally, expression of restores the phenotypes of the mutant. All velvet proteins heterologously expressed in are localized to the nuclear fraction independent of light. These data highlight the conservation of the complex formation in and However, they also underline the intergenera similarities and differences of velvet roles according to different life styles, niches and ontogenetic processes.
ISSN:	1943-2631 0016-6731 1943-2631