Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance

The recent developments of transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) have expanded plant breeding technology. One technical issue related to the current genome editing pro...

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Bibliographic Details
Published in:Plant Biotechnology 2016/12/25, Vol.33(5), pp.403-407
Main Authors: Numata, Keiji, Horii, Yoko, Motoda, Yoko, Hirai, Narumi, Nishitani, Chikako, Watanabe, Satoru, Kigawa, Takanori, Kodama, Yutaka
Format: Article
Language:English
Subjects:
apple
carrier peptide
neomycin phosphotransferase II (NPTII)
protein delivery
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Summary:The recent developments of transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) have expanded plant breeding technology. One technical issue related to the current genome editing process is residual transgenes for TALEN and CRISPR/Cas9 left in plant genomes after the editing process. Here, we aim to add transient kanamycin resistance into apple leaf cells by introducing neomycin phosphotransferase II (NPTII) into apple leaf cells using the fusion peptide system. At 75 mg/L of kanamycin for 2 days, apple JM1 leaf cells infiltrated with NPTII could be selected. Thus, we successfully demonstrated the first transient selection system of plant cells using a fusion peptide-mediated protein delivery system.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.16.0929a