Characterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches

Bacteria of the genus Paracoccus inhabit various pristine and anthropologically-shaped environments. Many Paracoccus spp. have biotechnological value and several are opportunistic human pathogens. Despite extensive knowledge of their metabolic potential and genome architecture, little is known about...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7899, Article 7899
Main Authors: Decewicz, Przemyslaw, Dziewit, Lukasz, Golec, Piotr, Kozlowska, Patrycja, Bartosik, Dariusz, Radlinska, Monika
Format: Article
Language:English
Subjects:
Bacteria
Computer Simulation
DNA, Viral - genetics
DNA, Viral - isolation & purification
Genome, Viral - genetics
Genomes
Genomics
Microscopy, Electron, Transmission
Mitomycin - pharmacology
Mitomycin C
Molecular Sequence Annotation
Mosaicism
Myoviridae - genetics
Myoviridae - isolation & purification
Myoviridae - ultrastructure
Opportunist infection
Paracoccus - drug effects
Paracoccus - genetics
Paracoccus - virology
Phages
Prophages
Prophages - drug effects
Prophages - genetics
Prophages - isolation & purification
Siphoviridae - genetics
Siphoviridae - isolation & purification
Siphoviridae - ultrastructure
Viruses
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Summary:Bacteria of the genus Paracoccus inhabit various pristine and anthropologically-shaped environments. Many Paracoccus spp. have biotechnological value and several are opportunistic human pathogens. Despite extensive knowledge of their metabolic potential and genome architecture, little is known about viruses of Paracoccus spp. So far, only three active phages infecting these bacteria have been identified. In this study, 16 Paracoccus strains were screened for the presence of active temperate phages, which resulted in the identification of five novel viruses. Mitomycin C-induced prophages were isolated, visualized and their genomes sequenced and thoroughly analyzed, including functional validation of their toxin-antitoxin systems. This led to the identification of the first active Myoviridae phage in Paracoccus spp. and four novel Siphoviridae phages. In addition, another 53 prophages were distinguished in silico within genomic sequences of Paracoccus spp. available in public databases. Thus, the Paracoccus virome was defined as being composed of 66 (pro)phages. Comparative analyses revealed the diversity and mosaicism of the (pro)phage genomes. Moreover, similarity networking analysis highlighted the uniqueness of Paracoccus (pro)phages among known bacterial viruses.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44460-4