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Treatment with the Pseudomonas aeruginosa Glycoside Hydrolase PslG Combats Wound Infection by Improving Antibiotic Efficacy and Host Innate Immune Activity
is an opportunistic, nosocomial bacterial pathogen that forms persistent infections due to the formation of protective communities, known as biofilms. Once the biofilm is formed, the bacteria embedded within it are recalcitrant to antimicrobial treatment and host immune defenses. Moreover, the prese...
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Published in: | Antimicrobial agents and chemotherapy 2019-06, Vol.63 (6) |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | is an opportunistic, nosocomial bacterial pathogen that forms persistent infections due to the formation of protective communities, known as biofilms. Once the biofilm is formed, the bacteria embedded within it are recalcitrant to antimicrobial treatment and host immune defenses. Moreover, the presence of biofilms in wounds is correlated with chronic infection and delayed healing. The current standard of care for chronic wound infections typically involves physical disruption of the biofilm via debridement and subsequent antimicrobial treatment. The glycoside hydrolases PelA
and PslG
have been demonstrated
to disrupt biofilm integrity through degradation of the key biofilm matrix exopolysaccharides Pel and Psl, respectively. Herein, we demonstrate that PslG
hydrolase therapy is a promising strategy for controlling
wound infections. Hydrolase treatment of
biofilms resulted in increased antibiotic efficacy and penetration into the biofilm. PslG
treatment of
biofilms also improved innate immune activity leading to greater complement deposition, neutrophil phagocytosis, and neutrophil reactive oxygen species production. Furthermore, when
-infected wounds were treated with a combination of PslG
and tobramycin, we observed an additive effect leading to greater bacterial clearance than treatments of tobramycin or PslG
alone. This study demonstrates that PelA
and PslG
have promising therapeutic potential and that PslG
may aid in the treatment of
wound infections. |
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ISSN: | 0066-4804 1098-6596 |
DOI: | 10.1128/AAC.00234-19 |