OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in Rice

Tillering and panicle branching are important determinants of plant architecture and yield potential in rice ( ). ( ) encodesSQUAMOSA PROMOTER BINDING PROTEIN-LIKE14, which acts as a key transcription factor regulating tiller outgrowth and panicle branching by directly activating the expression of (...

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Published in:The Plant cell 2019-05, Vol.31 (5), p.1026-1042
Main Authors: Duan, Erchao, Wang, Yihua, Li, Xiaohui, Lin, Qibing, Zhang, Ting, Wang, Yupeng, Zhou, Chunlei, Zhang, Huan, Jiang, Ling, Wang, Jiulin, Lei, Cailin, Zhang, Xin, Guo, Xiuping, Wang, Haiyang, Wan, Jianmin
Format: Article
Language:English
Subjects:
DNA-Binding Proteins
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant - genetics
Mutation
Oryza - anatomy & histology
Oryza - genetics
Oryza - growth & development
Phenotype
Plant Proteins - genetics
Plant Proteins - metabolism
Promoter Regions, Genetic - genetics
Protein Domains
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Tillering and panicle branching are important determinants of plant architecture and yield potential in rice ( ). ( ) encodesSQUAMOSA PROMOTER BINDING PROTEIN-LIKE14, which acts as a key transcription factor regulating tiller outgrowth and panicle branching by directly activating the expression of ( ) and ( ), thereby influencing grain yield in rice. Here, we report the identification of a rice mutant named that is characterized by dramatically reduced tiller number, enhanced culm strength, and increased panicle branch number. Map-based cloning revealed that ( ) encodes a plant-specific transcription factor of the SHI family with a characteristic family-specific IGGH domain and a conserved zinc-finger DNA binding domain. Consistent with the mutant phenotype, is predominantly expressed in axillary buds and young panicle, and its encoded protein is exclusively targeted to the nucleus. We show that OsSHI1 physically interacts with IPA1 both in vitro and in vivo. Moreover, OsSHI1 could bind directly to the promoter regions of both and through a previously unrecognized cis-element (T/GCTCTAC motif). OsSHI1 repressed the transcriptional activation activity of IPA1 by affecting its DNA binding activity toward the promoters of both and , resulting in increased tiller number and diminished panicle size. Taken together, our results demonstrate that OsSHI1 regulates plant architecture through modulating the transcriptional activity of IPA1 and provide insight into the establishment of plant architecture in rice.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.19.00023