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Regulating response and leukocyte adhesion of human endothelial cell by gradient nanohole substrate
Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gr...
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| Published in: | Scientific reports 2019-05, Vol.9 (1), p.7272, Article 7272 |
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| creator | Huang, Li-Hua Cui, Long-Hui Kim, Dae Hwan Joo, Hyung Joon Seo, Ha-Rim Choi, Seung-Cheol Noh, Ji-Min Lee, Kyu Back Hong, Soon Jun |
| description | Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gradient hole substrate. We examined the cellular response of human umbilical vein endothelial cells (HUVECs) by using a gradient nanohole substrate (GHS) with three different types of nanohole patterns (HP): which diameters were described in HP1, 120–200 nm; HP2, 200–280 nm; HP3, 280–360 nm. In results, HP2 GHS increased the attachment and proliferation of HUVECs. Also, gene expression of focal adhesion markers in HUVECs was significantly increased on HP2 GHS.
In vitro
tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. To summarize, optimal proliferations with reduced leukocyte adhesion of HUVECs were achieved by gradient nanohole substrate with 200–280 nm-sized holes. |
| doi_str_mv | 10.1038/s41598-019-43573-0 |
| format | article |
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In vitro
tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. To summarize, optimal proliferations with reduced leukocyte adhesion of HUVECs were achieved by gradient nanohole substrate with 200–280 nm-sized holes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-43573-0</identifier><identifier>PMID: 31086227</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/2 ; 13/21 ; 14/1 ; 14/19 ; 38 ; 38/77 ; 631/61/350/1058 ; 631/80/79/2066 ; Adhesion ; Basement Membrane - metabolism ; Blotting, Western ; Cell Adhesion ; Cytokines - metabolism ; Endothelial cells ; Fluorescent Antibody Technique ; Gene expression ; Human Umbilical Vein Endothelial Cells - metabolism ; Humanities and Social Sciences ; Humans ; In Situ Nick-End Labeling ; Leukocytes - metabolism ; multidisciplinary ; Nanopores - ultrastructure ; Reverse Transcriptase Polymerase Chain Reaction ; Science ; Science (multidisciplinary) ; Substrates ; Tissue engineering ; Umbilical vein</subject><ispartof>Scientific reports, 2019-05, Vol.9 (1), p.7272, Article 7272</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-ea3b76f81318050bfb87b819981a3b1b4186568afc99698ecece53b0c79fc86b3</citedby><cites>FETCH-LOGICAL-c511t-ea3b76f81318050bfb87b819981a3b1b4186568afc99698ecece53b0c79fc86b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2224343409/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2224343409?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,730,783,787,888,25765,27936,27937,37024,44602,53804,53806,75454</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31086227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Li-Hua</creatorcontrib><creatorcontrib>Cui, Long-Hui</creatorcontrib><creatorcontrib>Kim, Dae Hwan</creatorcontrib><creatorcontrib>Joo, Hyung Joon</creatorcontrib><creatorcontrib>Seo, Ha-Rim</creatorcontrib><creatorcontrib>Choi, Seung-Cheol</creatorcontrib><creatorcontrib>Noh, Ji-Min</creatorcontrib><creatorcontrib>Lee, Kyu Back</creatorcontrib><creatorcontrib>Hong, Soon Jun</creatorcontrib><title>Regulating response and leukocyte adhesion of human endothelial cell by gradient nanohole substrate</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gradient hole substrate. We examined the cellular response of human umbilical vein endothelial cells (HUVECs) by using a gradient nanohole substrate (GHS) with three different types of nanohole patterns (HP): which diameters were described in HP1, 120–200 nm; HP2, 200–280 nm; HP3, 280–360 nm. In results, HP2 GHS increased the attachment and proliferation of HUVECs. Also, gene expression of focal adhesion markers in HUVECs was significantly increased on HP2 GHS.
In vitro
tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. 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Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gradient hole substrate. We examined the cellular response of human umbilical vein endothelial cells (HUVECs) by using a gradient nanohole substrate (GHS) with three different types of nanohole patterns (HP): which diameters were described in HP1, 120–200 nm; HP2, 200–280 nm; HP3, 280–360 nm. In results, HP2 GHS increased the attachment and proliferation of HUVECs. Also, gene expression of focal adhesion markers in HUVECs was significantly increased on HP2 GHS.
In vitro
tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. To summarize, optimal proliferations with reduced leukocyte adhesion of HUVECs were achieved by gradient nanohole substrate with 200–280 nm-sized holes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31086227</pmid><doi>10.1038/s41598-019-43573-0</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 13/2 13/21 14/1 14/19 38 38/77 631/61/350/1058 631/80/79/2066 Adhesion Basement Membrane - metabolism Blotting, Western Cell Adhesion Cytokines - metabolism Endothelial cells Fluorescent Antibody Technique Gene expression Human Umbilical Vein Endothelial Cells - metabolism Humanities and Social Sciences Humans In Situ Nick-End Labeling Leukocytes - metabolism multidisciplinary Nanopores - ultrastructure Reverse Transcriptase Polymerase Chain Reaction Science Science (multidisciplinary) Substrates Tissue engineering Umbilical vein |
| title | Regulating response and leukocyte adhesion of human endothelial cell by gradient nanohole substrate |
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