Regulating response and leukocyte adhesion of human endothelial cell by gradient nanohole substrate

Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gr...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7272, Article 7272
Main Authors: Huang, Li-Hua, Cui, Long-Hui, Kim, Dae Hwan, Joo, Hyung Joon, Seo, Ha-Rim, Choi, Seung-Cheol, Noh, Ji-Min, Lee, Kyu Back, Hong, Soon Jun
Format: Article
Language:English
Subjects:
13/2
13/21
14/1
14/19
38
38/77
631/61/350/1058
631/80/79/2066
Adhesion
Basement Membrane - metabolism
Blotting, Western
Cell Adhesion
Cytokines - metabolism
Endothelial cells
Fluorescent Antibody Technique
Gene expression
Human Umbilical Vein Endothelial Cells - metabolism
Humanities and Social Sciences
Humans
In Situ Nick-End Labeling
Leukocytes - metabolism
multidisciplinary
Nanopores - ultrastructure
Reverse Transcriptase Polymerase Chain Reaction
Science
Science (multidisciplinary)
Substrates
Tissue engineering
Umbilical vein
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Summary:Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gradient hole substrate. We examined the cellular response of human umbilical vein endothelial cells (HUVECs) by using a gradient nanohole substrate (GHS) with three different types of nanohole patterns (HP): which diameters were described in HP1, 120–200 nm; HP2, 200–280 nm; HP3, 280–360 nm. In results, HP2 GHS increased the attachment and proliferation of HUVECs. Also, gene expression of focal adhesion markers in HUVECs was significantly increased on HP2 GHS. In vitro tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. To summarize, optimal proliferations with reduced leukocyte adhesion of HUVECs were achieved by gradient nanohole substrate with 200–280 nm-sized holes.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-43573-0