Genome-wide characterization of ALDH Superfamily in Brassica rapa and enhancement of stress tolerance in heterologous hosts by BrALDH7B2 expression

Aldehyde dehydrogenase (ALDH) carries out oxidation of toxic aldehydes using NAD /NADP as cofactors. In the present study, we performed a genome-wide identification and expression analysis of genes in the ALDH gene family in Brassica rapa. A total of 23 ALDH genes in the superfamily have been identi...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7012, Article 7012
Main Authors: Gautam, Ranjana, Ahmed, Israr, Shukla, Pawan, Meena, Rajesh Kumar, Kirti, P B
Format: Article
Language:English
Subjects:
Abiotic stress
Aldehyde dehydrogenase
Aldehyde Dehydrogenase - genetics
Aldehyde Dehydrogenase - metabolism
ALDH gene
Brassica rapa
Brassica rapa - enzymology
Brassica rapa - genetics
Chromosome Mapping
Chromosomes, Plant - genetics
Cofactors
Crop improvement
Drought
E coli
Escherichia coli - genetics
Escherichia coli - growth & development
Gene Expression Regulation, Plant
Genes
Genomes
Multigene Family
NAD
NADP
Overexpression
Oxidation
Plant Leaves - enzymology
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stems - enzymology
Plant Stems - genetics
Salinity tolerance
Stress, Physiological
Whole Genome Sequencing - methods
Yeasts - genetics
Yeasts - growth & development
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Summary:Aldehyde dehydrogenase (ALDH) carries out oxidation of toxic aldehydes using NAD /NADP as cofactors. In the present study, we performed a genome-wide identification and expression analysis of genes in the ALDH gene family in Brassica rapa. A total of 23 ALDH genes in the superfamily have been identified according to the classification of ALDH Gene Nomenclature Committee (AGNC). They were distributed unevenly across all 10 chromosomes. All the 23 Brassica rapa ALDH (BrALDH) genes exhibited varied expression patterns during treatments with abiotic stress inducers and hormonal treatments. The relative expression profiles of ALDH genes in B. rapa showed that they are predominantly expressed in leaves and stem suggesting their function in the vegetative tissues. BrALDH7B2 showed a strong response to abiotic stress and hormonal treatments as compared to other ALDH genes; therefore, it was overexpressed in heterologous hosts, E. coli and yeast to study its possible function under abiotic stress conditions. Over-expression of BrALDH7B2 in heterologous systems, E. coli and yeast cells conferred significant tolerance to abiotic stress treatments. Results from this work demonstrate that BrALDH genes are a promising and untapped genetic resource for crop improvement and could be deployed further in the development of drought and salinity tolerance in B. rapa and other economically important crops.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-43332-1