EDTA-Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Metallo-β-Lactamase-Producing Enterobacteriaceae

The increase in the prevalence and impact of infections caused by carbapenemase-producing is a global health concern. Therefore, rapid and accurate methods to detect these organisms in any clinical microbiology laboratory, including those in resource-limited settings, are essential to prevent and co...

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Bibliographic Details
Published in:Journal of clinical microbiology 2019-05, Vol.57 (5)
Main Authors: Sfeir, M M, Hayden, J A, Fauntleroy, K A, Mazur, C, Johnson, J K, Simner, P J, Das, S, Satlin, M J, Jenkins, S G, Westblade, L F
Format: Article
Language:English
Subjects:
Bacteriology
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Summary:The increase in the prevalence and impact of infections caused by carbapenemase-producing is a global health concern. Therefore, rapid and accurate methods to detect these organisms in any clinical microbiology laboratory, including those in resource-limited settings, are essential to prevent and contain their spread. It is also important to differentiate between serine- and metal-dependent carbapenemases elaborated by carbapenemase-producing isolates for epidemiologic, infection control and prevention, and therapeutic purposes. Here, we describe the development and evaluation of the EDTA-modified carbapenem inactivation method (eCIM), an assay for discriminating between serine- and metal-dependent (i.e., metallo-β-lactamases [MBLs]) carbapenemases when used in conjunction with the modified carbapenem inactivation method (mCIM). The eCIM had an overall sensitivity and specificity of 100% and was adopted by the Clinical and Laboratory Standards Institute as a method to use in combination with the mCIM to identify MBL-producing .
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01757-18