Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells

Objectives We prepared 3D poly (ε‐caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs). Materials and methods 3D nanofibres were prepared using a special collector for common electrospinning; simultaneo...

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Bibliographic Details
Published in:Cell proliferation 2013-02, Vol.46 (1), p.23-37
Main Authors: Rampichová, M., Chvojka, J., Buzgo, M., Prosecká, E., Mikeš, P., Vysloužilová, L., Tvrdík, D., Kochová, P., Gregor, T., Lukáš, D., Amler, E.
Format: Article
Language:English
Subjects:
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Differentiation
Cell Movement
Cell Proliferation
Cell Survival
Cells, Cultured
Elasticity
Humans
Integrin-Binding Sialoprotein - metabolism
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Nanofibers - chemistry
Nanofibers - ultrastructure
Original
Osteocalcin - metabolism
Osteogenesis
Polyesters - chemistry
Regenerative Medicine
Surface Properties
Tissue Engineering
Tissue Scaffolds
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Summary:Objectives We prepared 3D poly (ε‐caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs). Materials and methods 3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated. Results The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (P 
ISSN:0960-7722
1365-2184
DOI:10.1111/cpr.12001