Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies

We tested the contribution of nucleoside triphosphate (NTP) hydrolysis, ethanol, and organic acid syntheses, and H+-pump ATPases activity in the acidosis of anoxic sycamore (Acer pseudoplatanus) plant cells. Culture cells were chosen to alter NTP pools and fermentation with specific nutrient media (...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2001-02, Vol.125 (2), p.912-925
Main Authors: Gout, Elisabeth, Anne-Marie Boisson, Aubert, Serge, Douce, Roland, Bligny, Richard
Format: Article
Language:English
Subjects:
Acer - cytology
Acer - physiology
Adenosine triphosphatases
Agronomy. Soil science and plant productions
Anaerobiosis
Anoxia
Biological and medical sciences
Carbon Radioisotopes - metabolism
Cell membranes
Corn
Culture Media
Cytoplasm - physiology
Economic plant physiology
Environmental Stress and Adaptation
Ethanol
Fermentation
Fundamental and applied biological sciences. Psychology
Glycerol - metabolism
Hydrogen-Ion Concentration
Kinetics
Lactates
Magnetic Resonance Spectroscopy - methods
Metabolism
Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia)
Nutrition. Photosynthesis. Respiration. Metabolism
Phosphorus - metabolism
Photosynthesis, respiration. Anabolism, catabolism
Plant cells
Plant physiology and development
Plants
Radioisotope Dilution Technique
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Summary:We tested the contribution of nucleoside triphosphate (NTP) hydrolysis, ethanol, and organic acid syntheses, and H+-pump ATPases activity in the acidosis of anoxic sycamore (Acer pseudoplatanus) plant cells. Culture cells were chosen to alter NTP pools and fermentation with specific nutrient media (phosphate [Pi]-deprived and adenine- or glycerol-supplied). In vivo 31P- and 13C-nuclear magnetic resonance (NMR) spectroscopy was utilized to noninvasively measure intracellular pHs, Pi, phosphomonoesters, nucleotides, lactate, and ethanol. Following the onset of anoxia, cytoplasmic (cyt) pH (7.5) decreased to 6.8 within 4 to 5 min, whereas vacuolar pH (5.7) and external pH (6.5) remained stable. The NTP pool simultaneously decreased from 210 to
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.125.2.912