Functional Evaluation of Two Corneal Endothelial Cell-Based Therapies: Tissue-Engineered Construct and Cell Injection

Restoration of vision due to corneal blindness from corneal endothelial dysfunction can be achieved via a corneal transplantation. However, global shortage of donor tissues has driven the development cell-based therapeutics. With the capacity to propagate regulatory compliant human corneal endotheli...

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Bibliographic Details
Published in:Scientific reports 2019-04, Vol.9 (1), p.6087-6087, Article 6087
Main Authors: Peh, Gary S L, Ong, Hon Shing, Adnan, Khadijah, Ang, Heng-Pei, Lwin, Chan N, Seah, Xin-Yi, Lin, Shu-Jun, Mehta, Jodhbir S
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Animals
Blindness
Blindness - etiology
Blindness - therapy
Cells, Cultured
Child
Child, Preschool
Cornea
Corneal Edema - complications
Corneal Edema - pathology
Corneal Edema - therapy
Corneal transplantation
Corneal Transplantation - methods
Disease Models, Animal
Drug development
Endothelial cells
Endothelial Cells - transplantation
Endothelium, Corneal - cytology
Endothelium, Corneal - pathology
Female
Humans
Immunohistochemistry
Injection
Injections, Intraocular
Male
Middle Aged
Primary Cell Culture
Rabbits
Recovery of function
Tissue Engineering
Transplantation, Heterologous
Young Adult
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Summary:Restoration of vision due to corneal blindness from corneal endothelial dysfunction can be achieved via a corneal transplantation. However, global shortage of donor tissues has driven the development cell-based therapeutics. With the capacity to propagate regulatory compliant human corneal endothelial cells (CEnCs), this study evaluated the functionality of propagated CEnCs delivered via tissue-engineered endothelial keratoplasty (TE-EK) or corneal endothelial cell injection (CE-CI) within a rabbit model of bullous keratopathy. For animals with TE-EK grafts, central corneal thickness (CCT) increased to >1000 μm post-operatively. Gradual thinning with improvements in corneal clarity was observed from week 1. CCT at week 3 was 484.3 ± 73.7 μm. In rabbits with CE-CI, corneal clarity was maintained throughout, and CCT at week 3 was 582.5 ± 171.5 μm. Control corneas remained significantly edematous throughout the study period compared to their respective experimental groups (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-42493-3