Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway

Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation of tubulin can occur in the nucleus. So far, the potential function of nuclear tubulin has remained elusive. In this work, we show that α- and β-tubul...

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Bibliographic Details
Published in:Scientific reports 2019-04, Vol.9 (1), p.5725-5725, Article 5725
Main Authors: Schwarzerová, K, Bellinvia, E, Martinek, J, Sikorová, L, Dostál, V, Libusová, L, Bokvaj, P, Fischer, L, Schmit, A C, Nick, P
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - physiology
Animal models
Animals
Biochemistry, Molecular Biology
Biology
Botanics
Cancer
Cell division
Cell Line
Cell Nucleus - metabolism
Cloning
Cytoplasm
Cytoplasm - metabolism
Eukaryotic Cells - metabolism
Exportin 1 Protein
Exports
Humans
Interphase
Karyopherins - metabolism
Leptomycin B
Life Sciences
Microscopy
Microtubules
Microtubules - metabolism
Mutagenesis
Nicotiana - metabolism
Nuclear transport
Physiology
Plant cells
Protein Transport - physiology
Proteins
Receptors, Cytoplasmic and Nuclear - metabolism
Tubulin
Tubulin - metabolism
Vegetal Biology
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Summary:Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation of tubulin can occur in the nucleus. So far, the potential function of nuclear tubulin has remained elusive. In this work, we show that α- and β-tubulins of various organisms contain multiple conserved nuclear export sequences, which are potential targets of the Exportin 1/CRM1 pathway. We demonstrate exemplarily that these NES motifs are sufficient to mediate export of GFP as model cargo and that this export can be inhibited by leptomycin B, an inhibitor of the Exportin 1/CRM1 pathway. Likewise, leptomycin B causes accumulation of GFP-tagged tubulin in interphase nuclei, in both plant and animal model cells. Our analysis of nuclear tubulin content supports the hypothesis that an important function of nuclear tubulin export is the exclusion of tubulin from interphase nuclei, after being trapped by nuclear envelope reassembly during telophase.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-42056-6