Investigating supramolecular systems using Förster resonance energy transfer

Supramolecular systems have applications in areas as diverse as materials science, biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems can be challenging due to the wide range of time scales, binding strengths, distances, and concentrations at which non-covalent phe...

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Bibliographic Details
Published in:Chemical Society reviews 2018-09, Vol.47 (18), p.727-744
Main Authors: Teunissen, Abraham J. P, Pérez-Medina, Carlos, Meijerink, Andries, Mulder, Willem J. M
Format: Article
Language:English
Subjects:
Analytical chemistry
Drug delivery systems
Energy transfer
Fluorescence Resonance Energy Transfer
In vivo methods and tests
Kinetics
Macromolecular Substances - chemical synthesis
Macromolecular Substances - chemistry
Macromolecular Substances - metabolism
Materials science
Measurement methods
Nanoparticles
Organic chemistry
Proteins
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Summary:Supramolecular systems have applications in areas as diverse as materials science, biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems can be challenging due to the wide range of time scales, binding strengths, distances, and concentrations at which non-covalent phenomena take place. Due to their versatility and sensitivity, Förster resonance energy transfer (FRET)-based techniques are excellently suited to meet such challenges. Here, we detail the ways in which FRET has been used to study non-covalent interactions in both synthetic and biological supramolecular systems. Among other topics, we examine methods to measure molecular forces, determine protein conformations, monitor assembly kinetics, and visualize in vivo drug release from nanoparticles. Furthermore, we highlight multiplex FRET techniques, discuss the field's limitations, and provide a perspective on new developments. This review details the ways Förster resonance energy transfer (FRET) can be used to study natural and synthetic supramolecular systems.
ISSN:0306-0012
1460-4744
1460-4744
DOI:10.1039/c8cs00278a