The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression

Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the...

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Bibliographic Details
Published in:Scientific reports 2019-03, Vol.9 (1), p.5314-12, Article 5314
Main Authors: Melnikova, Larisa, Elizar'ev, Pavel, Erokhin, Maksim, Molodina, Varvara, Chetverina, Darya, Kostyuchenko, Margarita, Georgiev, Pavel, Golovnin, Anton
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Computational Biology
Deoxyribonucleic acid
DNA
DNA-Binding Proteins
Drosophila - genetics
Drosophila - metabolism
Drosophila Proteins - chemistry
Drosophila Proteins - metabolism
Enhancer Elements, Genetic
Gene Expression Regulation
Gene silencing
Insulator Elements
Oocytes
Promoter Regions, Genetic
Protein Binding
Protein Interaction Domains and Motifs
Proteins
Repressor Proteins - chemistry
Repressor Proteins - metabolism
Zinc finger proteins
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Summary:Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described in vivo function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720-892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw).
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-41761-6