The Expression of the SLIT–ROBO Family in Adult Patients with Acute Myeloid Leukemia

Introduction SLIT–ROBO is a ligand–receptor family of neuronal guidance cues that has been involved in pathological and physiological angiogenesis. SLIT–ROBO expression is altered in many tumours. However, no data exist about the role of the whole family in acute myelogenous myeloid leukemia (AML)....

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Bibliographic Details
Published in:Archivum Immunologiae et Therapiae Experimentalis 2019-04, Vol.67 (2), p.109-123
Main Authors: Gołos, Aleksandra, Jesionek-Kupnicka, Dorota, Gil, Lidia, Braun, Marcin, Komarnicki, Mieczyslaw, Robak, Tadeusz, Wierzbowska, Agnieszka
Format: Article
Language:English
Subjects:
Acute myeloid leukemia
Adolescent
Adult
Aged
Aged, 80 and over
Angiogenesis
Axon Guidance
Biomedical and Life Sciences
Biomedicine
Biopsy
Bone marrow
Cancer
CD34 antigen
Female
Gene Expression Regulation, Neoplastic
Genomes
Humans
Immunohistochemistry
Immunology
Intercellular Signaling Peptides and Proteins - genetics
Intercellular Signaling Peptides and Proteins - metabolism
Leukemia
Leukemia, Myeloid, Acute - immunology
Male
Membrane Proteins - genetics
Membrane Proteins - metabolism
Middle Aged
Myeloid leukemia
Neovascularization, Pathologic
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Original
Original Article
Paraffin
Pharmacology/Toxicology
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Ribonucleic acid
Risk groups
RNA
Robo protein
Roundabout Proteins
Slit protein
Tumors
Young Adult
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Summary:Introduction SLIT–ROBO is a ligand–receptor family of neuronal guidance cues that has been involved in pathological and physiological angiogenesis. SLIT–ROBO expression is altered in many tumours. However, no data exist about the role of the whole family in acute myelogenous myeloid leukemia (AML). Purpose Herein, we assessed the expression of all SLIT–ROBO family in bone marrow (BM) biopsy of AML patients and control group on both protein and RNA levels. Methods The paraffin-embedded tissue blocks were subjected to immunohistochemistry for SLIT1, SLIT2, SLIT3, ROBO1, ROBO2, ROBO3, and ROBO4. Microvessel density (MVD) was evaluated by CD34 immunohistochemistry. An in silico analysis using The Cancer Genome Atlas data repository was conducted for assessment of RNA level. Results Acute myeloid leukemia patients were generally high expressers of ROBO1 and ROBO2 compared to the controls ( p  
ISSN:0004-069X
1661-4917
DOI:10.1007/s00005-019-00535-8