Long Chain Non-Coding RNA (lncRNA) HOTAIR Knockdown Increases miR-454-3p to Suppress Gastric Cancer Growth by Targeting STAT3/Cyclin D1

BACKGROUND Gastric cancer is a common gastrointestinal tumor. The incidence and mortality of gastric cancer are very high. Therefore, it is important to study targeted drugs. Recent studies found long chain non-coding RNA (lncRNAs) and microRNAs (miRNAs) were abnormal in gastric cancer. MATERIAL AND...

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Published in:Medical science monitor 2019-02, Vol.25, p.1537-1548
Main Authors: Jiang, Datong, Li, He, Xiang, Heping, Gao, Ming, Yin, Chunlin, Wang, Haiping, Sun, Yuansong, Xiong, Maoming
Format: Article
Language:English
Subjects:
Apoptosis - genetics
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation - genetics
Cyclin D1 - genetics
Cyclin D1 - metabolism
Gene Knockdown Techniques
Genes, bcl-1
Humans
Lab/In Vitro Research
MicroRNAs - genetics
MicroRNAs - metabolism
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
STAT3 Transcription Factor - genetics
STAT3 Transcription Factor - metabolism
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
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Summary:BACKGROUND Gastric cancer is a common gastrointestinal tumor. The incidence and mortality of gastric cancer are very high. Therefore, it is important to study targeted drugs. Recent studies found long chain non-coding RNA (lncRNAs) and microRNAs (miRNAs) were abnormal in gastric cancer. MATERIAL AND METHODS We collected adjacent normal and cancer tissues of gastric cancer patients and measured HOTAIR, miR-454-3p, STAT3, and Cyclin D1 expression and analyzed the correlation with clinical status. We also measured AGS and SGC7901 cells proliferation rate of different groups by MTT assay, and we evaluated AGS and SGC7901 cell apoptosis and cell cycle by flow cytometry. In addition, we assessed the relative proteins expressions by WB assay. Finally, we explored the correlation between miR-454-3p and STAT3 by use of double luciferase reporter. RESULTS lncRNA HOTAIR was negatively correlated with miR-454-3p expression in gastric cancer tissues. lncRNA HOTAIR knockdown suppressed AGS and SGC7901, which are gastric cancer cell lines that promote cell proliferation by increasing cell apoptosis and keeping the cell cycle in G1 phase. In further mechanism research, we found that the STAT3 and Cyclin D1 proteins expressions were suppressed by lncRNA HOTAIR down-regulation in AGS and SGC7901 cells. CONCLUSIONS Our results suggest that lncRNA HOTAIR knockdown stimulates miR-454-3p expression to inhibit gastric cancer growth by depressing STAT3/Cyclin D1 activity.
ISSN:1643-3750
1234-1010
1643-3750
DOI:10.12659/MSM.913087