Effect of Age, High-Fat Diet, and Rat Strain on Serum Biomarkers and Telomere Length and Global DNA Methylation in Peripheral Blood Mononuclear Cells

The objective of the current study was to determine if age, diet, and genetic disposition (animal strain) in an animal model had early effects on specific molecular markers in circulating peripheral blood mononuclear cells (PBMCs). Three strains [Sprague-Dawley (SD), Fischer 344 (F344), and Brown-No...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-02, Vol.9 (1), p.1996-1996, Article 1996
Main Authors: Antonini, James M, Kodali, Vamsi, Meighan, Terence G, Roach, Katherine A, Roberts, Jenny R, Salmen, Rebecca, Boyce, Greg R, Zeidler-Erdely, Patti C, Kashon, Michael, Erdely, Aaron, Shoeb, Mohammad
Format: Article
Language:English
Subjects:
Age
Body weight gain
DNA methylation
High fat diet
Inhalation
Leukocytes (mononuclear)
Long-term effects
Occupational exposure
Peripheral blood mononuclear cells
Rodents
Smoke
Tobacco
Triglycerides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of the current study was to determine if age, diet, and genetic disposition (animal strain) in an animal model had early effects on specific molecular markers in circulating peripheral blood mononuclear cells (PBMCs). Three strains [Sprague-Dawley (SD), Fischer 344 (F344), and Brown-Norway (BN)] of male rats were maintained on a high-fat (HF) or regular diet. Blood was collected at 4, 12, and 24 wk to assess chemistry and to recover PBMCs. Triglycerides and body weight gain increased at all time points in the HF diet group for each strain. Telomere length in PBMCs decreased in the HF diet group compared to the regular diet group up to 24 wk in all strains. Telomere length decreased in PBMCs at 24 wk compared to baseline in all strains, indicating an age-related effect. These findings highlight that diet and age cause changes in PBMCs recovered from different strains of rats. The next tier of studies will examine the contribution of an occupational exposure (e.g., welding fume inhalation) in combination with diet, age, and strain, to assess changes in the molecular responses of isolated PBMCs. In addition, studies involving lifestyle exposure (e.g., tobacco smoke) are in the planning stages and will assess the long-term effects of exposure in our animal model.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-38192-0