Breast cancer resistance protein (BCRP) gene expression in a cohort of adult Egyptian patients with acute myeloid leukemia

Acute myeloid leukemia (AML), an aggressive clonal disease, is genetically heterozygous. The prognostic role of expression of Breast Cancer Resistance Protein (BCRP) gene, which behaves as a multidrug transporter, in adult AML is ambiguous. The objective is to assess the level of mRNA expression of...

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Bibliographic Details
Published in:African health sciences 2018-12, Vol.18 (4), p.958-964
Main Authors: El-Masry, Manal W, Gouda, Heba M, Shaheen, Iman A, Edesa, Wael, Hassan, Naglaa M, Ramzy, Rehab
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age Factors
Aged
Antigens, CD34 - biosynthesis
Antineoplastic Combined Chemotherapy Protocols - administration & dosage
Antineoplastic Combined Chemotherapy Protocols - adverse effects
Antineoplastic Combined Chemotherapy Protocols - therapeutic use
ATP Binding Cassette Transporter, Subfamily G, Member 2 - biosynthesis
Comorbidity
Egypt
Female
Humans
Leukemia, Myeloid, Acute - drug therapy
Leukemia, Myeloid, Acute - genetics
Leukemia, Myeloid, Acute - pathology
Male
Middle Aged
Neoplasm Proteins - biosynthesis
Prognosis
Real-Time Polymerase Chain Reaction
RNA, Messenger
Young Adult
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Summary:Acute myeloid leukemia (AML), an aggressive clonal disease, is genetically heterozygous. The prognostic role of expression of Breast Cancer Resistance Protein (BCRP) gene, which behaves as a multidrug transporter, in adult AML is ambiguous. The objective is to assess the level of mRNA expression of BCRP gene in newly diagnosed cytogenetically normal adult Egyptian AML patients; and to clarify its potential influence and association between therapeutic responsiveness and disease free survival. The BCRP gene expression was evaluated by quantifying its mRNA using real time RT-PCR in fifty newly diagnosed cytogenetically normal adult AML patients and 20 healthy normal controls. The expression was evaluated in relation to clinical and prognostic factors, response to treatment and the survival rate. BCRP mRNA was over expressed in adult AML patients compared to controls. This study showed a positive statistical correlation between BCRP gene expression and the percent of CD34 expression. Statistical analysis did not reveal any association between BCRP expression level and chemotherapeutic responsiveness or disease free survival rate. The significance of BCRP gene expression and its function in AML is very complicated, therefore more standardized clinical studies are needed.
ISSN:1680-6905
1729-0503
1680-6905
DOI:10.4314/ahs.v18i4.15