Gcd Gene Diversity of Quinoprotein Glucose Dehydrogenase in the Sediment of Sancha Lake and Its Response to the Environment

Quinoprotein glucose dehydrogenase (GDH) is the most important enzyme of inorganic phosphorus-dissolving metabolism, catalyzing the oxidation of glucose to gluconic acid. The insoluble phosphate in the sediment is converted into soluble phosphate, facilitating mass reproduction of algae. Therefore,...

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Bibliographic Details
Published in:International journal of environmental research and public health 2018-12, Vol.16 (1), p.1
Main Authors: Li, Yong, Zhang, Jianqiang, Gong, Zhiliang, Xu, Wenlai, Mou, Zishen
Format: Article
Language:English
Subjects:
Annual precipitation
Bacteria
Carbon
China
Cloning
Creeks
Cyanobacteria
Dehydrogenases
Deoxyribonucleic acid
DNA
Enzymes
Eutrophication
Eutrophication - physiology
Gene amplification
Gene expression
Genetic Variation
Geologic Sediments - microbiology
Glucose
Glucose dehydrogenase
Glucose Dehydrogenases - genetics
Humid climates
Lake sediments
Lakes
Lakes - microbiology
Metabolism
Metabolites
Microorganisms
Nitrogen
Organic acids
Phosphates
Phosphorus
Phosphorus - metabolism
Pollutants
Pollution control
Potassium
Proteobacteria - genetics
Rainfall
Rivers
Seasons
Sediment distribution
Sediments
Water inflow
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Summary:Quinoprotein glucose dehydrogenase (GDH) is the most important enzyme of inorganic phosphorus-dissolving metabolism, catalyzing the oxidation of glucose to gluconic acid. The insoluble phosphate in the sediment is converted into soluble phosphate, facilitating mass reproduction of algae. Therefore, studying the diversity of genes which encode GDH is beneficial to reveal the microbial group that has a significant influence on the eutrophication of water. Taking the eutrophic Sancha Lake sediments as the research object, we acquired samples from six sites in the spring and autumn. A total of 219,778 high-quality sequences were obtained by DNA extraction of microbial groups in sediments, PCR amplification of the gene, and high-throughput sequencing. Six phyla, nine classes, 15 orders, 29 families, 46 genera, and 610 operational taxonomic units (OTUs) were determined, suggesting the high genetic diversity of . genes came mainly from the genera of (1.63⁻77.99%), (0.13⁻56.95%), (0.32⁻25.49%), and (0.16⁻11.88%) in the phylum of Proteobacteria (25.10⁻98.85%). The abundance of these dominant -harboring bacteria was higher in the spring than in autumn, suggesting that they have an important effect on the eutrophication of the Sancha Lake. The alpha and beta diversity of genes presented spatial and temporal differences due to different sampling site types and sampling seasons. Pearson correlation analysis and canonical correlation analysis (CCA) showed that the diversity and abundance of genes were significantly correlated with environmental factors such as dissolved oxygen (DO), phosphorus hydrochloride (HCl⁻P), and dissolved total phosphorus (DTP). OTU composition was significantly correlated with DO, total organic carbon (TOC), and DTP. GDH encoded by genes transformed insoluble phosphate into dissolved phosphate, resulting in the eutrophication of Sancha Lake. The results suggest that genes encoding GDH may play an important role in lake eutrophication.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph16010001