Small Molecule Chelators Reveal That Iron Starvation Inhibits Late Stages of Bacterial Cytokinesis

Bacterial cell division requires identification of the division site, assembly of the division machinery, and constriction of the cell envelope. These processes are regulated in response to several cellular and environmental signals. Here, we use small molecule iron chelators to characterize the sur...

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Bibliographic Details
Published in:ACS chemical biology 2018-01, Vol.13 (1), p.235-246
Main Authors: Santos, Thiago M. A, Lammers, Matthew G, Zhou, Maoquan, Sparks, Ian L, Rajendran, Madhusudan, Fang, Dong, De Jesus, Crystal L. Y, Carneiro, Gabriel F. R, Cui, Qiang, Weibel, Douglas B
Format: Article
Language:English
Subjects:
Bacteria - cytology
Bacteria - drug effects
Bacteria - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Benzimidazoles - metabolism
Benzimidazoles - pharmacology
Caulobacter crescentus - cytology
Caulobacter crescentus - drug effects
Caulobacter crescentus - metabolism
Cobalt - pharmacology
Copper - pharmacology
Cytokinesis - drug effects
Escherichia coli - cytology
Escherichia coli - drug effects
Escherichia coli - metabolism
Gene Expression Regulation, Bacterial - drug effects
Hydrazines - metabolism
Hydrazines - pharmacology
Iron - metabolism
Iron - pharmacology
Iron Chelating Agents - metabolism
Iron Chelating Agents - pharmacology
Naphthalenes - metabolism
Naphthalenes - pharmacology
Peptidoglycan - metabolism
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Summary:Bacterial cell division requires identification of the division site, assembly of the division machinery, and constriction of the cell envelope. These processes are regulated in response to several cellular and environmental signals. Here, we use small molecule iron chelators to characterize the surprising connections between bacterial iron homeostasis and cell division. We demonstrate that iron starvation downregulates the transcription of genes encoding proteins involved in cell division, reduces protein biosynthesis, and prevents correct positioning of the division machinery at the division site. These combined events arrest the constriction of the cell during late stages of cytokinesis in a manner distinct from known mechanisms of inhibiting cell division. Overexpression of genes encoding cell division proteins or iron transporters partially suppresses the biological activity of iron chelators and restores growth and division. We propose a model demonstrating the effect of iron availability on the regulatory mechanisms coordinating division in response to the nutritional state of the cell.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.7b00560