Microarray-based technologies for the discovery of selective, RNA-binding molecules

•Microarrays are an invaluable tool to discover and study RNA-binding molecules.•Peptide, peptoid, aminoglycoside, and small molecule microarrays are covered.•Likely impact of these technologies on the development of RNA-targeted therapeutics is discussed. The identification of small molecules that...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2016-07, Vol.103, p.188-195
Main Authors: Abulwerdi, Fardokht A., Schneekloth, John S.
Format: Article
Language:English
Subjects:
Aminoglycosides
Base Sequence
Drug Evaluation, Preclinical
Gene Expression Regulation
Humans
Inverted Repeat Sequences
Microarrays
Molecular Targeted Therapy
Oligonucleotide Array Sequence Analysis
Peptides
Peptoids
RNA
Small molecules
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Summary:•Microarrays are an invaluable tool to discover and study RNA-binding molecules.•Peptide, peptoid, aminoglycoside, and small molecule microarrays are covered.•Likely impact of these technologies on the development of RNA-targeted therapeutics is discussed. The identification of small molecules that bind specifically to RNA is a challenge. However, the recent explosion in knowledge about the role RNA plays in a number of physiological processes apart from coding for protein sequences makes it a highly interesting target for chemical probes and therapeutics. One technology that has played an important role in the discovery of RNA-binding molecules is microarrays. Microarrays have been broadly employed to screen, profile, and quantify RNA interactions, and will likely play an important role in the discovery of new classes of ligands going forward. Here, we discuss the development of microarray technologies, including aminoglycoside, peptide, peptoid, and small molecule microarrays, and their use in studying RNA-interacting molecules.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2016.04.022